ArticleArchaea (Vancouver, B.C.)2008
New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species.
Article in Archaea (Vancouver, B.C.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
90 citing papers in PubMed, 149 citations in OpenAlex.
- Methanol-specific methyltransferase isozymes have large carbon kinetic isotope effects that impact methane isotopic signatures.Applied and environmental microbiology · 2026Article
- Component A2 is a redox-sensitive archaeal ATPase activated by methyl-coenzyme M reductase.Current biology : CB · 2026Article
- A family of archaeal hibernation factors that bind in tandem and protect ribosomes in dormant cells.Nature communications · 2026Article
- Valine overproduction inMetabolic engineering communications · 2026Article
- Metabolic engineering ofMetabolic engineering communications · 2026Article
- Component A2 is a redox-sensitive archaeal ATPase activated by methyl-coenzyme M reductase.bioRxiv : the preprint server for biology · 2026Article
- Genus-specific remodeling of carbon and energy metabolism facilitates acetoclastic methanogenesis inJournal of bacteriology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- A family of archaeal hibernation factors that bind in tandem and protect ribosomes in dormant cells.bioRxiv : the preprint server for biology · 2026Article
- Removal and bypass of restriction modification systems increases transformation efficiency ofFrontiers in microbiology · 2026Article
- Overexpression of 2-mercaptoethanesulfonate biosynthesis genesJournal of bacteriology · 2025Article
- Methanogenic archaea encoding Pyrrolysine maintain ambiguous amber codon usage.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genus-specific remodeling of carbon and energy metabolism facilitates acetoclastic methanogenesis inbioRxiv : the preprint server for biology · 2025Article
- Mer overexpression inApplied and environmental microbiology · 2025Article
- Methanogenesis marker 16 metalloprotein is the primary coenzyme M synthase in Methanosarcina acetivorans.PLoS genetics · 2025Article
- Article
- Production of the Sesquiterpene Bisabolene From One- and Two-Carbon Compounds in Engineered Methanosarcina acetivorans.Microbial biotechnology · 2025Article
- Structural organization of pyruvate: ferredoxin oxidoreductase from the methanogenic archaeon Methanosarcina acetivorans.Structure (London, England : 1993) · 2024Article
- Uncovering the small proteome of Methanosarcina mazei using Ribo-seq and peptidomics under different nitrogen conditions.Nature communications · 2024Article
- A promoter-RBS library for fine-tuning gene expression inApplied and environmental microbiology · 2024Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A highly efficient method for chromosomal integration of cloned DNA into Methanosarcina spp. was developed utilizing the site-specific recombination system from the Streptomyces phage phiC31. Host strains expressing the phiC31 integrase gene and carrying an appropriate recombination site can be transformed with non-replicating plasmids carrying the complementary recombination site at efficiencies similar to those obtained with self-replicating vectors. We have also constructed a series of hybrid promoters that combine the highly expressed M. barkeri PmcrB promoter with binding sites for the tetracycline-responsive, bacterial TetR protein. These promoters are tightly regulated by the presence or absence of tetracycline in strains that express the tetR gene. The hybrid promoters can be used in genetic experiments to test gene essentiality by placing a gene of interest under their control. Thus, growth of strains with tetR-regulated essential genes becomes tetracycline-dependent. A series of plasmid vectors that utilize the site-specific recombination system for construction of reporter gene fusions and for tetracycline regulated expression of cloned genes are reported. These vectors were used to test the efficiency of translation at a variety of start codons. Fusions using an ATG start site were the most active, whereas those using GTG and TTG were approximately one half or one fourth as active, respectively. The CTG fusion was 95% less active than the ATG fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.