ArticleVeterinary immunology and immunopathology2009
Development of monoclonal antibodies to detect bovine FOXP3 in PBMCs exposed to a staphylococcal superantigen.
Article in Veterinary immunology and immunopathology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- Live-attenuated foot-and-mouth disease virus vaccine engineered by codon deoptimization induces a strong protective immune response in cattle.NPJ vaccines · 2026Article
- Interplay of Regulatory T Cell and Th17 Cells during Infectious Diseases in Humans and Animals.Frontiers in immunology · 2017Review
- Characterization and expression of monoclonal antibody-defined molecules on resting and activated bovine αβ, γδ T and NK cells.Veterinary immunology and immunopathology · 2015Article
- Immunity, safety and protection of an Adenovirus 5 prime--Modified Vaccinia virus Ankara boost subunit vaccine against Mycobacterium avium subspecies paratuberculosis infection in calves.Veterinary research · 2014Article
- Bovine γδ T cells are a major regulatory T cell subset.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Deletion of relA abrogates the capacity of Mycobacterium avium paratuberculosis to establish an infection in calves.Frontiers in cellular and infection microbiology · 2014Article
- Immunohistochemical investigation of Foxp3 expression in the intestine in healthy and diseased dogs.Veterinary research · 2012Article
- Crohn's disease and Mycobacterium avium subsp. paratuberculosis: the need for a study is long overdue.Veterinary immunology and immunopathology · 2012Review
- Superantigen-mediated differentiation of bovine monocytes into dendritic cells.Journal of leukocyte biology · 2009Article
- The immunopathology of sepsis: pathogen recognition, systemic inflammation, the compensatory anti-inflammatory response, and regulatory T cells.Journal of veterinary internal medicineReview
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The role of regulatory T cells (Tregs) is well documented in immune homeostasis and protection against autoimmune disease. Forkhead box protein 3 (FOXP3) has been shown to be essential for the development and function of T(reg). Due to the lack of tools for FOXP3 detection in certain species, understanding the role of Treg in a variety of ruminant diseases has been hampered. In this study, we developed monoclonal antibodies (mAbs) against bovine FOXP3 using recombinant bovine FOXP3 lacking the forkhead domain as an immunogen. The specificity of the mAbs was confirmed by immunoblot and mass spectrometry. Expression of FOXP3 was induced in bovine PBMCs after 6 d of exposure to staphylococcal enterotoxin type C1 (SEC1) in vitro. Similar to findings in mice and humans, expression of FOXP3 was restricted to CD4+ CD25+ T cells. Transcriptional analysis of bovine TCR variable regions of the beta chain (boVbeta) showed that transcription of boVbeta sequences reactive with SEC1 increased for 6 d, and then boVbeta sequences non-reactive with SEC1 rapidly increased in the cultures. This indicates that induction of FOXP3+ CD4+ CD25+ Tregs by SEC1 is not Vbeta restricted. The FOXP3 mAbs developed in this study will be useful in the further investigation of the role of Treg in staphylococcal pathogenesis in bovine mastitis and other ruminant diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.