ArticleJournal of the American Chemical Society2008
Photostable single-molecule nanoparticle optical biosensors for real-time sensing of single cytokine molecules and their binding reactions.
Article in Journal of the American Chemical Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed.
- Quantifying Citrate Surface Ligands on Iron Oxide Nanoparticles with TGA, CHN Analysis, NMR, and RP-HPLC with UV Detection.Analytical chemistry · 2025Article
- Antimicrobial peptide-conjugated phage-mimicking nanoparticles exhibit potent bactericidal action againstNanoscale advances · 2024Article
- Development of Nano-Micro Fused LSPR Chip for In Situ Single-Cell Secretion Analysis.Micromachines · 2023Article
- Toxic Effects of Silver Ions on Early Developing Zebrafish Embryos Distinguished from Silver Nanoparticles.ACS omega · 2022Article
- Biocompatible, Multi-Mode, Fluorescent,Pharmaceuticals (Basel, Switzerland) · 2022Article
- Oxidative Stress and Apoptotic Responses Elicited byCancers · 2020Article
- Size-Dependent Inhibitory Effects of Antibiotic Nanocarriers on Filamentation ofNanoscale advances · 2020Article
- Article
- Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology.Micromachines · 2020Article
- Single Cell Analysis of Neutrophils NETs by Microscopic LSPR Imaging System.Micromachines · 2019Article
- Chemical Sensors Based on Two-Dimensional (2D) Materials for Selective Detection of Ions and Molecules in Liquid.Frontiers in chemistry · 2019Review
- An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation.Lab on a chip · 2018Article
- Review
- Single gold nanoparticle plasmonic spectroscopy for study of chemical-dependent efflux function of single ABC transporters of single live Bacillus subtilis cells.The Analyst · 2018Article
- Article
- Emerging Cytokine Biosensors with Optical Detection Modalities and Nanomaterial-Enabled Signal Enhancement.Sensors (Basel, Switzerland) · 2017Review
- Single Nanoparticle Plasmonic Spectroscopy for Study of Charge-Dependent Efflux Function of Multidrug ABC Transporters of Single LiveThe journal of physical chemistry. C, Nanomaterials and interfaces · 2016Article
- Electrophoretic Separation of Single Particles Using Nanoscale Thermoplastic Columns.Analytical chemistry · 2016Article
- Label-free cytokine micro- and nano-biosensing towards personalized medicine of systemic inflammatory disorders.Advanced drug delivery reviews · 2015Review
- Wavelength dependent specific plasmon resonance coupling of single silver nanoparticles with EGFP.Nanoscale · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We synthesized tiny stable silver nanoparticles (2.6 +/- 1.1 nm) and used its small surface area and functional groups to control single molecule detection (SMD) volumes on single nanoparticles. These new approaches allowed us to develop intrinsic single molecule nanoparticle optical biosensors (SMNOBS) for sensing and imaging of single human cytokine molecules, recombinant human tumor necrosis factor-alpha (TNFalpha), and probing its binding reaction with single monoclonal antibody (MAB) molecules in real-time. We found that SMNOBS retained their biological activity over months and showed exceptionally high photostability. Our study illustrated that smaller nanoparticles exhibited higher dependence of optical properties on surface functional groups, making it a much more sensitive biosensor. Localized surface plasmon resonance spectra (LSPRS) of SMNOBS showed a large red shift of peak wavelength of 29 +/- 11 nm, as single TNFalpha molecules bound with single MAB molecules on single nanoparticles. Utilizing its LSPRS, we quantitatively measured its binding reaction in real time at single molecule (SM) level, showing stochastic binding kinetics of SM reactions with binding equilibrium times ranging from 30 to 120 min. SMNOBS exhibited extraordinarily high sensitivity and selectivity, and a notably wide dynamic range of 0-200 ng/mL (0-11.4 nM). Thus, SMNOBS is well suited for the fundamental study of biological functions of single protein molecules and SM interactions of chemical functional groups with the surface of nanoparticles, as well as development of effective disease diagnosis and therapy.
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