ArticleJournal of the American Chemical Society2008
The role of electrostatics in siderophore recognition by the immunoprotein Siderocalin.
Article in Journal of the American Chemical Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 57 citations in OpenAlex.
- Actinium chelation and crystallization in a macromolecular scaffold.Nature communications · 2024Article
- Iron Acquisition by Bacterial Pathogens: Beyond Tris-Catecholate Complexes.Chembiochem : a European journal of chemical biology · 2020Review
- The iron load of lipocalin-2 (LCN-2) defines its pro-tumour function in clear-cell renal cell carcinoma.British journal of cancer · 2020Article
- Experimental and theoretical structural/spectroscopical correlation of enterobactin and catecholamide.Data in brief · 2018Article
- Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAMScientific reports · 2017Article
- Lipocalin 2 alleviates iron toxicity by facilitating hypoferremia of inflammation and limiting catalytic iron generation.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2016Article
- Coordination Chemistry of Microbial Iron Transport.Accounts of chemical research · 2015Article
- Purification and Structural Characterization of "Simple Catechol", the NGAL-Siderocalin Siderophore in Human Urine.RSC advances · 2015Article
- EGCG inhibit chemical reactivity of iron through forming an Ngal-EGCG-iron complex.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2013Article
- Siderocalin outwits the coordination chemistry of vibriobactin, a siderophore of Vibrio cholerae.ACS chemical biology · 2013Article
- Siderocalins: Siderophore binding proteins evolved for primary pathogen host defense.Current opinion in chemical biology · 2013Review
- Mammalian siderophores, siderophore-binding lipocalins, and the labile iron pool.The Journal of biological chemistry · 2012Review
- Siderocalin/Lcn2/NGAL/24p3 does not drive apoptosis through gentisic acid mediated iron withdrawal in hematopoietic cell lines.PloS one · 2012Article
- Immune interference in Mycobacterium tuberculosis intracellular iron acquisition through siderocalin recognition of carboxymycobactins.ACS chemical biology · 2011Article
- Galline Ex-FABP is an antibacterial siderocalin and a lysophosphatidic acid sensor functioning through dual ligand specificities.Structure (London, England : 1993) · 2011Article
- The v-myc-induced Q83 lipocalin is a siderocalin.The Journal of biological chemistry · 2010Article
- Iron traffics in circulation bound to a siderocalin (Ngal)-catechol complex.Nature chemical biology · 2010Article
- The Staphylococcus aureus siderophore receptor HtsA undergoes localized conformational changes to enclose staphyloferrin A in an arginine-rich binding pocket.The Journal of biological chemistry · 2010Article
- Neutrophil gelatinase-associated lipocalin expresses antimicrobial activity by interfering with L-norepinephrine-mediated bacterial iron acquisition.Antimicrobial agents and chemotherapy · 2010Article
- Microbial iron acquisition: marine and terrestrial siderophores.Chemical reviews · 2009Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Iron is required for virulence of most bacterial pathogens, many of which rely on siderophores, small-molecule chelators, to scavenge iron in mammalian hosts. As an immune response, the human protein Siderocalin binds both apo and ferric siderophores in order to intercept delivery of iron to the bacterium, impeding virulence. The introduction of steric clashes into the siderophore structure is an important mechanism of evading sequestration. However, in the absence of steric incompatibilities, electrostatic interactions determine siderophore strength of binding by Siderocalin. By using a series of isosteric enterobactin analogues, the contribution of electrostatic interactions, including both charge-charge and cation-pi, to the recognition of 2,3-catecholate siderophores has been deconvoluted. The analogues used in the study incorporate a systematic combination of 2,3-catecholamide (CAM) and N-hydroxypyridinonate (1,2-HOPO) binding units on a tris(2-aminoethyl)amine (tren) backbone, [tren(CAM)(m)(1,2-HOPO)(n), where m = 0, 1, 2, or 3 and n = 3 - m]. The shape complementarity of the synthetic analogue series was determined through small-molecule crystallography, and the binding interactions were investigated through a fluorescence-based binding assay. These results were modeled and correlated through ab initio calculations of the electrostatic properties of the binding units. Although all the analogues are accommodated in the binding pocket of Siderocalin, the ferric complexes incorporating decreasing numbers of CAM units are bound with decreasing affinities (K(d) = >600, 43, 0.8, and 0.3 nM for m = 0-3). These results elucidate the role of electrostatics in the mechanism of siderophore recognition by Siderocalin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.