ArticleBioconjugate chemistry2008
Site-specific, thiol-mediated conjugation of fluorescent probes to cysteine-modified diabodies targeting CD20 or HER2.
Article in Bioconjugate chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- IgG from Dermatophagoides pteronyssinus (Der p)-atopic individuals modulates non-atopic thymic B cell phenotype (alfa-4/beta-7) and cytokine production (IFN-γ, IL-9, and IL-10) with direct membrane interaction.Scientific reports · 2024Article
- Development of Anti-LRRC15 Small Fragments for Imaging Purposes Using a Phage-Display ScFv Approach.International journal of molecular sciences · 2022Article
- Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging.Molecular imaging and biology · 2019Article
- Template-Catalyzed, Disulfide Conjugation of Monoclonal Antibodies Using a Natural Amino Acid Tag.Bioconjugate chemistry · 2018Article
- Construction of homogeneous antibody-drug conjugates using site-selective protein chemistry.Chemical science · 2016Review
- Site-Specifically Labeled Immunoconjugates for Molecular Imaging--Part 1: Cysteine Residues and Glycans.Molecular imaging and biology · 2016Review
- A fully human scFv phage display library for rapid antibody fragment reformatting.Protein engineering, design & selection : PEDS · 2015Article
- Article
- Anti-MET immunoPET for non-small cell lung cancer using novel fully human antibody fragments.Molecular cancer therapeutics · 2014Article
- Enhanced immunoPET of ALCAM-positive colorectal carcinoma using site-specific ⁶⁴Cu-DOTA conjugation.Protein engineering, design & selection : PEDS · 2014Article
- Design and preclinical evaluation of a 99mTc-labelled diabody of mAb J591 for SPECT imaging of prostate-specific membrane antigen (PSMA).EJNMMI research · 2014Article
- An engineered cysteine-modified diabody for imaging activated leukocyte cell adhesion molecule (ALCAM)-positive tumors.Molecular imaging and biology · 2012Article
- ImmunoPET using engineered antibody fragments: fluorine-18 labeled diabodies for same-day imaging.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2012Article
- Multimodal interventional molecular imaging of tumor margins and distant metastases by targeting αvβ3 integrin.Chembiochem : a European journal of chemical biology · 2012Article
- Protein-based tumor molecular imaging probes.Amino acids · 2011Review
- Site-specific conjugation of monodispersed DOTA-PEGn to a thiolated diabody reveals the effect of increasing peg size on kidney clearance and tumor uptake with improved 64-copper PET imaging.Bioconjugate chemistry · 2011Article
- Fluorescent triphenyl substituted maleimide derivatives: synthesis, spectroscopy and quantum chemical calculations.Journal of fluorescence · 2010Article
- Antibody vectors for imaging.Seminars in nuclear medicine · 2010Review
- ImmunoPET imaging of B-cell lymphoma using 124I-anti-CD20 scFv dimers (diabodies).Protein engineering, design & selection : PEDS · 2010Article
- Cys-diabody quantum dot conjugates (immunoQdots) for cancer marker detection.Bioconjugate chemistry · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Small, engineered antibody fragments such as diabodies (50 kDa noncovalent dimers of single-chain Fv fragments) are useful alternatives to their larger antibody counterparts. However, due to their size, they are more susceptible to disruption of their antigen binding sites when modified using random conjugation techniques. Previous work has demonstrated the utility of a C-terminal cysteine modification for site-specific radiolabeling of an anti-CEA diabody, resulting in the creation of a cys-diabody (CysDb). In the present work, the adaptability of the CysDb system was explored by creating two additional CysDbs: one specific for CD20 and one for HER2. Purified CysDbs of both specificities demonstrated behavior consistent with stable, covalent dimers harboring a readily reducible disulfide bond. Each CysDb was site-specifically conjugated to three different fluorophores for optical detection: the large fluorescent proteins phycoerythrin (PE) and allophycocyanin (APC), and the small fluorescent molecule Alexa Fluor488. Fluorophore-conjugated CysDbs bound specifically to their targets in both antigen systems and with each different fluorescent tag as determined by flow cytometry. In vitro specific antigen binding was observed in the presence of a mixture of specific and nonspecifically conjugated CysDbs. Conjugates retained both specificity and fluorescence, demonstrating the successful expansion of the CysDb repertoire to new targets and to new site-specific conjugation possibilities.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.