ArticleHistochemistry and cell biology2009
Vinculin and cellular retinol-binding protein-1 are markers for quiescent and activated hepatic stellate cells in formalin-fixed paraffin embedded human liver.
Article in Histochemistry and cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 52 citations in OpenAlex.
- Galectin-3 in biliary atresia and other pediatric cholestatic liver diseases.Hepatology research : the official journal of the Japan Society of Hepatology · 2024Article
- Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Enhancement of Zyxin Promotes Skin Fibrosis by Regulating FAK/PI3K/AKT and TGF-β Signaling Pathways via Integrins.International journal of biological sciences · 2023Article
- Characterization of focal adhesion proteins in rodent hepatic stellate cells.Histochemistry and cell biology · 2022Article
- Single-cell RNA sequencing of human liver reveals hepatic stellate cell heterogeneity.JHEP reports : innovation in hepatology · 2021Article
- Hepatic stellate cell-specific knockout of transcriptional intermediary factor 1γ aggravates liver fibrosis.The Journal of experimental medicine · 2020Article
- Hepatic stellate cell hypertrophy is associated with metabolic liver fibrosis.Scientific reports · 2020Article
- Article
- Effects of AM80 compared to AC261066 in a high fat diet mouse model of liver disease.PloS one · 2019Article
- Expression, purification, and evaluation of in vivo anti-fibrotic activity for soluble truncated TGF-β receptor II as a cleavable His-SUMO fusion protein.World journal of microbiology & biotechnology · 2018Article
- Stellate cells and mesenchymal stem cells in benign mammary stroma are associated with risk factors for breast cancer - an observational study.BMC cancer · 2018Observational
- Human hepatic stellate cell isolation and characterization.Journal of gastroenterology · 2018Review
- Identification of markers for quiescent pancreatic stellate cells in the normal human pancreas.Histochemistry and cell biology · 2017Article
- Reelin expression in human liver of patients with chronic hepatitis C infection.European journal of histochemistry : EJH · 2017Article
- The development of hepatic stellate cells in normal and abnormal human fetuses - an immunohistochemical study.Physiological reports · 2015Article
- Adiponectin modulates focal adhesion disassembly in activated hepatic stellate cells: implication for reversing hepatic fibrosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Regulator of G-protein signaling-5 is a marker of hepatic stellate cells and expression mediates response to liver injury.PloS one · 2014Article
- Inflammation rapidly modulates the expression of ALDH1A1 (RALDH1) and vimentin in the liver and hepatic macrophages of rats in vivo.Nutrition & metabolism · 2014Article
- Syncoilin is an intermediate filament protein in activated hepatic stellate cells.Histochemistry and cell biology · 2014Article
- Clinical significance and gene expression study of human hepatic stellate cells in HBV related-hepatocellular carcinoma.Journal of experimental & clinical cancer research : CR · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatic stellate cells (HSCs) have important roles in the pathogenesis of liver fibrosis and cirrhosis. As response to chronic injury HSCs are activated and change from quiescent into myofibroblast-like cells. Several HSC-specific markers have been described in rat or mouse models. The aim of our work was to identify the best marker(s) for human HSCs. To this end we used the automated high throughput NexES IHC staining device (Ventana Medical Systems) to incubate sections under standardized conditions. Formalin fixed paraffin embedded (FFPE) normal and diseased human livers were studied. With immunohistochemistry we examined the expression of synemin, desmin, vimentin, vinculin, neurotrophin-3 (NT-3), alpha-smooth muscle actin (alpha-SMA), cellular retinol-binding protein-1 (CRBP-1), glial fibrillary acidic protein (GFAP), cysteine- and glycine-rich protein 2 (CRP2), and cytoglobin/stellate cell activation-associated protein (cygb/STAP). This is the first study in which a series of HSC markers is compared on serial FFPE human tissues. CRBP-1 clearly stains lobular HSCs without reacting with smooth muscle cells (SMCs) and shows variable cholangiocyte positivity. Vinculin has a similar staining pattern as CRBP-1 but additionally stains SMCs, and (myo)fibroblasts. In conclusion, we therefore propose to use CRBP-1 and/or vinculin to stain HSCs in human liver tissues.
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