ArticleInternational journal of legal medicine2009
Forensic species identification based on size variation of mitochondrial DNA hypervariable regions.
Article in International journal of legal medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 28 citations in OpenAlex.
- Simple and field-adapted species identification of biological specimens combining multiplex multienzyme isothermal rapid amplification, lateral flow dipsticks, and universal primers for initial rapid screening without standard PCR laboratory.International journal of legal medicine · 2024Article
- Using capillary electrophoresis to identify Anopheline species in routine sampling sites.Ecology and evolution · 2024Article
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- Insertion-and-Deletion Mutations between the Genomes of SARS-CoV, SARS-CoV-2, and Bat Coronavirus RaTG13.Microbiology spectrum · 2022Article
- Development and validation of simultaneous identification of 26 mammalian and poultry species by a multiplex assay.International journal of legal medicine · 2022Article
- Current issues for mammalian species identification in forensic science: a review.International journal of legal medicine · 2021Review
- The genome profiling method can be applied for species identification of biological materials collected at crime scenes.BMC genetics · 2019Article
- Whole-Genome Re-Alignment Facilitates Development of Specific Molecular Markers for Races 1 and 4 of Xanthomonas campestris pv. campestris, the Cause of Black Rot Disease in Brassica oleracea.International journal of molecular sciences · 2017Article
- Brief communication: DNA from early Holocene American dog.American journal of physical anthropology · 2011Article
- Identification of species by multiplex analysis of variable-length sequences.Nucleic acids research · 2010Article
- Developing equine mtDNA profiling for forensic application.International journal of legal medicine · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, two new systems for species identification were developed based on size variation of mitochondrial DNA hypervariable regions among animals: one was a conventional method using non-fluorescent primer sets and agarose gel electrophoresis and the other was an automatic method using fluorescent primer sets and capillary electrophoresis. DNA samples from 18 mammal, four birds, and 19 fish species were amplified using three primer sets specific for mammals, birds, and fishes, respectively. The differences in the sizes of the polymerase chain reaction (PCR) products, ranging from about 350 to 900 bp, permitted us to identify species. These systems were successfully applied to various specimens from several criminal cases. In unknown samples, which were different in size from reference DNA markers, sequencing of the PCR products and subsequent BLAST analysis helped to identify species. Furthermore, the sequence data provided us with information on individuals. Because these species identification methods are very simple, easy, rapid, and exact, they are useful in the field of forensic science.
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