ArticleJournal of immunology (Baltimore, Md. : 1950)2008
Outgrowth of CD4low/negCD25+ T cells with suppressor function in CD4+CD25+ T cell cultures upon polyclonal stimulation ex vivo.
Article in Journal of immunology (Baltimore, Md. : 1950), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- Preservation of antigen-specific responses in cryopreserved CD4Journal of translational autoimmunity · 2022Article
- Small Heterodimer Partner Regulates Dichotomous T Cell Expansion by Macrophages.Endocrinology · 2019Article
- Article
- Development of Auto Antigen-specific Regulatory T Cells for Diabetes Immunotherapy.Immune network · 2016Review
- Stem Cell-Derived Regulatory T Cells for Therapeutic Use in Arthritis.Autoimmune and infectious diseases : open access · 2016Article
- FOXP3 expression is upregulated in CD4T cells in progressive HIV-1 infection and is a marker of disease severity.PloS one · 2010Article
- Helper T cells down-regulate CD4 expression upon chronic stimulation giving rise to double-negative T cells.Cellular immunologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
CD4(+)CD25(+) regulatory T cells (Tregs) play an essential role in controlling autoimmunity and allograft rejection. Several ex vivo activation and expansion protocols have been developed to amplify cell numbers and suppressor function of murine and human Tregs. We demonstrate in this study that ex vivo activation and expansion of murine Tregs resulted in an enrichment of a CD4(low/neg)CD25(+) T cell population that was more than 20-fold more potent than expanded conventional Tregs in suppressing an in vitro CD4(+)CD25(-) T cell response to allo-Ag. The generation of CD4(low/neg)CD25(+) T cells was independent of the presence of Tregs in the culture, and suppressor function was acquired only after activation and expansion. CD4(low/neg)CD25(+) T cells expressed either an alphabeta or gammadelta TCR, had an activated phenotype, and did not express the transcription factor FoxP3. Despite expressing the cell surface Ags lymphocyte activation gene-3 (CD223) and CD103, neither was essential for suppressor cell function. Suppression by CD4(low/neg)CD25(+) T cells was prevented by a semipermeable membrane and was independent of IL-10 and TGF-beta. In summary, we describe in this study CD4(low/neg)CD25(+) FoxP3(neg) T cells with highly potent suppressor cell function derived from cultures of an enriched population of CD4(+)CD25(+) T cells that may contribute to the suppressor activity of ex vivo expanded bone fide Tregs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.