ArticleJournal of immunology (Baltimore, Md. : 1950)2008
Lipoxin A4 counterregulates GM-CSF signaling in eosinophilic granulocytes.
Article in Journal of immunology (Baltimore, Md. : 1950), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Glucocorticoids, their uses, sexual dimorphisms, and diseases: new concepts, mechanisms, and discoveries.Physiological reviews · 2024Review
- Bioactive lipid-based therapeutic approach to COVID-19 and other similar infections.Archives of medical science : AMS · 2023Article
- Specialized pro-resolving lipid mediators in endodontics: a narrative review.BMC oral health · 2021Review
- Bioactive Lipids in COVID-19-Further Evidence.Archives of medical research · 2021Review
- Could Arachidonic Acid-Derived Pro-Resolving Mediators Be a New Therapeutic Strategy for Asthma Therapy?Frontiers in immunology · 2020Review
- Cytokine-Induced Glucocorticoid Resistance from Eosinophil Activation: Protein Phosphatase 5 Modulation of Glucocorticoid Receptor Phosphorylation and Signaling.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Key mechanisms governing resolution of lung inflammation.Seminars in immunopathology · 2016Review
- Lipoxin B₄ promotes the resolution of allergic inflammation in the upper and lower airways of mice.Mucosal immunology · 2015Article
- Altered eosinophil proteome in a patient with hypereosinophilia from acute fascioliasis.Clinical and vaccine immunology : CVI · 2011Article
- Specialized proresolving mediator targets for RvE1 and RvD1 in peripheral blood and mechanisms of resolution.The Biochemical journal · 2011Article
- Interleukin-13 signaling and its role in asthma.The World Allergy Organization journal · 2011Article
- Resolvins: natural agonists for resolution of pulmonary inflammation.Progress in lipid research · 2011Review
- Effect of lipoxin A₄ on IL-1β production of monocytes and its possible mechanism in severe preeclampsia.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2010Article
- Molecular circuits of resolution in airway inflammation.TheScientificWorldJournal · 2010Review
- Resolvin E1 receptor activation signals phosphorylation and phagocytosis.The Journal of biological chemistry · 2010Article
- Lipoxins: resolutionary road.British journal of pharmacology · 2009Review
- TGF-beta can leave you breathless.Current opinion in pharmacology · 2009Review
- The effect of Lipoxin A4 on the interaction between macrophage and osteoblast: possible role in the treatment of aseptic loosening.BMC musculoskeletal disorders · 2009Article
- Toward the Proteome of the Human Peripheral Blood Eosinophil.Proteomics. Clinical applications · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Eosinophils are granulated leukocytes that are involved in many inflammation-associated pathologies including airway inflammation in asthma. Resolution of eosinophilic inflammation and return to homeostasis is in part due to endogenous chemical mediators, for example, lipoxins, resolvins, and protectins. Lipoxins are endogenous eicosanoids that demonstrate antiinflammatory activity and are synthesized locally at sites of inflammation. In view of the importance of lipoxins (LXs) in resolving inflammation, we investigated the molecular basis of LXA(4) action on eosinophilic granulocytes stimulated with GM-CSF employing the eosinophilic leukemia cell line EoL-1 as well as peripheral blood eosinophils. We report herein that LXA(4) (1-100 nM) decreased protein tyrosine phosphorylation in EoL-1 cells stimulated with GM-CSF. Additionally, the expression of a number of GM-CSF-induced cytokines was inhibited by LXA(4) in a dose-dependent manner. Furthermore, using a proteomics approach involving mass spectrometry and immunoblot analysis we identified 11 proteins that were tyrosine phosphorylated after GM-CSF stimulation and whose phosphorylation was significantly inhibited by LXA(4) pretreatment. Included among these 11 proteins were alpha-fodrin (nonerythroid spectrin) and actin. Microscopic imaging showed that treatment of EoL-1 cells or blood eosinophils with GM-CSF resulted in the reorganization of actin and the translocation of alpha-fodrin from the cytoplasm to the plasma membrane. Importantly, alpha-fodrin translocation was prevented by LXA(4) but actin reorganization was not. Thus, the mechanism of LXA(4) action likely involves prevention of activation of eosinophilic granulocytes by GM-CSF through inhibition of protein tyrosine phosphorylation and modification of some cytoskeletal components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.