ArticleNucleic acids research2009
Mutagenic inverted repeat assisted genome engineering (MIRAGE).
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- A genetic interaction map centered on cohesin reveals auxiliary factors involved in sister chromatid cohesion inJournal of cell science · 2020Article
- Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli.Microbial cell factories · 2020Article
- Yeast genetic interaction screens in the age of CRISPR/Cas.Current genetics · 2019Review
- Recombineering inFrontiers in cellular and infection microbiology · 2017Article
- Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes.PloS one · 2017Article
- Article
- Size of gene specific inverted repeat--dependent gene deletion In Saccharomyces cerevisiae.PloS one · 2013Article
- A chemical approach to mapping nucleosomes at base pair resolution in yeast.Methods in enzymology · 2012Article
- GAL1-SceI directed site-specific genomic (gsSSG) mutagenesis: a method for precisely targeting point mutations in S. cerevisiae.BMC biotechnology · 2011Article
- Optimizing pentose utilization in yeast: the need for novel tools and approaches.Biotechnology for biofuels · 2010Article
- Tandem repeat coupled with endonuclease cleavage (TREC): a seamless modification tool for genome engineering in yeast.Nucleic acids research · 2010Article
- DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways.Nucleic acids research · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Here we describe a one-step method to create precise modifications in the genome of Saccharomyces cerevisiae as a tool for synthetic biology, metabolic engineering, systems biology and genetic studies. Through homologous recombination, a mutagenesis cassette containing an inverted repeat of selection marker(s) is integrated into the genome. Due to its inherent instability in genomic DNA, the inverted repeat catalyzes spontaneous self-excision, resulting in precise genome modification. Since this excision occurs at very high frequencies, selection for the integration event can be followed immediately by counterselection, without the need for growth in permissive conditions. This is the first time a truly one-step method has been described for genome modification in any organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.