ArticleNucleic acids research2009
DNAzyme-mediated catalysis with only guanosine and cytidine nucleotides.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Thermodynamic and Kinetic Sequence Selection in Enzyme-Free Polymer Self-Assembly inside a Non-equilibrium RNA Reactor.Life (Basel, Switzerland) · 2022Article
- A Novel Small RNA-Cleaving Deoxyribozyme with a Short Binding Arm.Scientific reports · 2019Article
- Side chain determinants of biopolymer function during selection and replication.Nature chemical biology · 2019Article
- Allosteric DNAzyme-based DNA logic circuit: operations and dynamic analysis.Nucleic acids research · 2019Article
- A comparison of genotype-phenotype maps for RNA and proteins.Biophysical journal · 2012Article
- Mismatch Discrimination and Efficient Photomodulation with Split 10-23 DNAzymes.Inorganica chimica acta · 2012Article
- In vitro selection of metal ion-selective DNAzymes.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Article
- A genotype-to-phenotype map of in vitro selected RNA-cleaving DNAzymes: implications for accessing the target phenotype.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-stranded DNA molecules have the capacity to adopt catalytically active structures known as DNAzymes, although the fundamental limits of this ability have not been determined. Starting with a parent DNAzyme composed of all four types of standard nucleotides, we conducted a search of the surrounding sequence space to identify functional derivatives with catalytic cores composed of only three, and subsequently only two types of nucleotides. We provide the first report of a DNAzyme that contains only guanosine and cytidine deoxyribonucleotides in its catalytic domain, which consists of just 13 nucleotides. This DNAzyme catalyzes the Mn(2+)-dependent cleavage of an RNA phosphodiester bond approximately 5300-fold faster than the corresponding uncatalyzed reaction, but approximately 10,000-fold slower than the parent. The demonstration of a catalytic DNA molecule made from a binary nucleotide alphabet broadens our understanding of the fundamental limits of nucleic-acid-mediated catalysis.
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Registered trials
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