ArticleThe Journal of biological chemistry2009
Phosphatidic acid and N-acylphosphatidylethanolamine form membrane domains in Escherichia coli mutant lacking cardiolipin and phosphatidylglycerol.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 82 citations in OpenAlex.
- FtsK with a unique N-terminal extension is involved in coordinating the final steps of chromosome segregation with asymmetric division in mycobacterial cells.Journal of bacteriology · 2026Article
- Phospholipid-independent biogenesis and function of the RP4 conjugation pilus.Nature communications · 2026Article
- Phospholipid-independent biogenesis of a functional RP4 conjugation pilus.bioRxiv : the preprint server for biology · 2025Article
- Molecular basis of cell membrane adaptation in daptomycin-resistant Enterococcus faecalis.JCI insight · 2024Article
- Comprehensive lipidomic analysis of the genusmSphere · 2024Article
- Biosynthesized gold nanoparticles that activate Toll-like receptors and elicit localized light-converting hyperthermia for pleiotropic tumor immunoregulation.Nature communications · 2023Article
- Construction of a Bacterial Lipidomics Analytical Platform: Pilot Validation with Bovine Paratuberculosis Serum.Metabolites · 2023Article
- Eugene P. Kennedy's Legacy: Defining Bacterial Phospholipid Pathways and Function.Frontiers in molecular biosciences · 2021Review
- Two-week administration of engineered Escherichia coli establishes persistent resistance to diet-induced obesity even without antibiotic pre-treatment.Applied microbiology and biotechnology · 2019Article
- Lipidomic consequences of phospholipid synthesis defects in Escherichia coli revealed by HILIC-ion mobility-mass spectrometry.Chemistry and physics of lipids · 2019Article
- Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.BMC biology · 2018Article
- Biogenesis, transport and remodeling of lysophospholipids in Gram-negative bacteria.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Tat transport in Escherichia coli requires zwitterionic phosphatidylethanolamine but no specific negatively charged phospholipid.FEBS letters · 2017Article
- Impact of Membrane Phospholipid Alterations in Escherichia coli on Cellular Function and Bacterial Stress Adaptation.Journal of bacteriology · 2017Article
- Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii.Scientific reports · 2017Article
- In Vivo and in Vitro Synthesis of Phosphatidylglycerol by an Escherichia coli Cardiolipin Synthase.The Journal of biological chemistry · 2016Article
- Quantitative analysis of N-acylphosphatidylethanolamine molecular species in rat brain using solid-phase extraction combined with reversed-phase chromatography and tandem mass spectrometry.Journal of separation science · 2016Article
- Negatively Charged Lipids as a Potential Target for New Amphiphilic Aminoglycoside Antibiotics: A BIOPHYSICAL STUDY.The Journal of biological chemistry · 2016Article
- System-Wide Adaptations of Desulfovibrio alaskensis G20 to Phosphate-Limited Conditions.PloS one · 2016Article
- The membrane: transertion as an organizing principle in membrane heterogeneity.Frontiers in microbiology · 2015Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The pgsA null Escherichia coli strain, UE54, lacks the major anionic phospholipids phosphatidylglycerol and cardiolipin. Despite these alterations the strain exhibits relatively normal cell division. Analysis of the UE54 phospholipids using negativeion electrospray ionization mass spectrometry resulted in identification of a new anionic phospholipid, N-acylphosphatidylethanolamine. Staining with the fluorescent dye 10-N-nonyl acridine orange revealed anionic phospholipid membrane domains at the septal and polar regions. Making UE54 null in minCDE resulted in budding off of minicells from polar domains. Analysis of lipid composition by mass spectrometry revealed that minicells relative to parent cells were significantly enriched in phosphatidic acid and N-acylphosphatidylethanolamine. Thus despite the absence of cardiolipin, which forms membrane domains at the cell pole and division sites in wild-type cells, the mutant cells still maintain polar/septal localization of anionic phospholipids. These three anionic phospholipids share common physical properties that favor polar/septal domain formation. The findings support the proposed role for anionic phospholipids in organizing amphitropic cell division proteins at specific sites on the membrane surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.