ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2008
Evaluation of tail biopsy collection in laboratory mice (Mus musculus): vertebral ossification, DNA quantity, and acute behavioral responses.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Evaluation of common anesthetic and analgesic techniques for tail biopsy in mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2012Trial
- Rectal Swab DNA Collection Protocol for PCR Genotyping in Rats.bioRxiv : the preprint server for biology · 2024Article
- Regulation of posterior Hox genes by sex steroids explains vertebral variation in inbred mouse strains.Journal of anatomy · 2022Review
- The Deletion of Hdac4 in Mouse Osteoblasts Influences Both Catabolic and Anabolic Effects in Bone.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2018Article
- An Efficient, Simple, and Noninvasive Procedure for Genotyping Aquatic and Nonaquatic Laboratory Animals.Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- Behavioral Characteristics of Adult Zebrafish (Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
- QuantitativeBone reports · 2016Article
- Effects of Topical Anesthetics on Behavior, Plasma Corticosterone, and Blood Glucose Levels after Tail Biopsy of C57BL/6NHSD Mice (Mus musculus).Journal of the American Association for Laboratory Animal Science : JAALAS · 2016Article
- Adverse effects of vapocoolant and topical anesthesia for tail biopsy of preweanling mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2015Article
- Peripheral nerve injury modulates neurotrophin signaling in the peripheral and central nervous system.Molecular neurobiology · 2014Review
- Developmental and behavioral effects of toe clipping on neonatal and preweanling mice with and without vapocoolant anesthesia.Journal of the American Association for Laboratory Animal Science : JAALAS · 2014Article
- An experimental model of partial insulin-like growth factor-1 deficiency in mice.Journal of physiology and biochemistry · 2014Article
- Sensory neuron development in mouse coccygeal vertebrae and its relationship to tail biopsies for genotyping.PloS one · 2014Article
- Local cryoanalgesia is effective for tail-tip biopsy in mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Article
- Behavioral and activity assessment of laboratory mice (Mus musculus) after tail biopsy under isoflurane anesthesia.Journal of the American Association for Laboratory Animal Science : JAALAS · 2011Article
- A system for implanting laboratory mice with light-activated microtransponders.Journal of the American Association for Laboratory Animal Science : JAALAS · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A preferred method to genotype genetically engineered mice is through collection of distal tail tissue (tail biopsy) followed by DNA isolation. Currently, general or local anesthesia (or both) is recommended for biopsy after 3 wk of age, the time after which tail vertebrae are considered to be ossified. Our objective was to rigorously evaluate vertebral development, DNA content, and acute behavioral responses at different ages by harvesting tail biopsies of different lengths. We evaluated laboratory mice from 5 inbred strains and 1 outbred stock at each of 12 ages (3 to 42 d of age). Biopsies of 5-, 10-, and 15-mm lengths were obtained. Vertebrae were graded according to level of ossification by using complementary modalities of high-resolution microradiography, microcomputed tomography, and histology. Vertebral development progressed at different rates among the strains, with mature tail vertebrae containing endplates detectable in the tail of some strains by 10 d of age. Within the distal 2 mm of tail, end plates were not identified before 21 d of age. DNA yield (DNA weight/tissue weight) was greatest from the 5-mm biopsy harvest. Acute behavioral responses to biopsy varied by age and strain, and these differences were associated with vertebral maturation. Vertebral development progressed most rapidly in C57BL/6 mice, which also demonstrated the highest response rate to biopsy, whereas BALB/c mice had slower vertebral development and were less responsive. These findings support the collection of minimal lengths of tail tissue from mice at ages younger than 17 d, unless anesthesia or analgesia is provided.
Indexed as
Identifiers
19049247PMC2687139W164584601What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.