ArticleApplied and environmental microbiology2009
Optimized method for preparation of DNA from pathogenic and environmental mycobacteria.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- The mycobacterial ATP burst is a lysis artifact and serves as an assay for drug-induced cell wall damage.Cell reports · 2026Article
- The mycobacterial ATP burst is a lysis artifact and serves as an assay for drug-induced cell wall damage.bioRxiv : the preprint server for biology · 2025Article
- Sudapyridine (WX-081) inhibitsmSphere · 2025Article
- Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow.Journal of visualized experiments : JoVE · 2025Article
- Turbolysis: A low-cost, small footprint alternative to commercial bead beaters for cell lysis.HardwareX · 2024Article
- Evaluation of an electricity-independent method for IS2404 Loop-mediated isothermal amplification (LAMP) diagnosis of Buruli ulcer in resource-limited settings.PLoS neglected tropical diseases · 2024Article
- Non-Microbiological Mycobacterial Detection Techniques for Quality Control of Biological Products: A Comprehensive Review.Microorganisms · 2024Review
- Comparison of nine extraction methods for bacterial identification using the ONT MinION sequencer.International journal of legal medicine · 2024Article
- Effects of dietary fiber and biotic supplementation on apparent total tract macronutrient digestibility and the fecal characteristics, metabolites, and microbiota of healthy adult dogs.Journal of animal science · 2024Article
- Genomic Epidemiological Analysis of Antimicrobial-Resistant Bacteria with Nanopore Sequencing.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Primary Isolation of Mycobacterium ulcerans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- High-Quality Nucleic Acid Isolation from Hard-to-Lyse Bacterial Strains Using PMAP-36, a Broad-Spectrum Antimicrobial Peptide.International journal of molecular sciences · 2021Article
- The Multiomics Analyses of Fecal Matrix and Its Significance to Coeliac Disease Gut Profiling.International journal of molecular sciences · 2021Review
- Characterization of integrated prophages within diverse species of clinical nontuberculous mycobacteria.Virology journal · 2020Article
- Complete nontuberculous mycobacteria whole genomes using an optimized DNA extraction protocol for long-read sequencing.BMC genomics · 2019Article
- TBAJ-876 Retains Bedaquiline's Activity against Subunits c and ε ofAntimicrobial agents and chemotherapy · 2019Article
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- Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment.PloS one · 2019Article
- Laboratory confirmation of Buruli ulcer cases in Ghana, 2008-2016.PLoS neglected tropical diseases · 2018Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genomic studies on pathogenic and environmental mycobacteria are of growing interest for understanding of their evolution, distribution, adaptation, and host-pathogen interaction. Since most mycobacteria are slow growers, material from in vitro cultures is usually scarce. The robust mycobacterial cell wall hinders both experimental cell lysis and efficient DNA extraction. Here, we compare elements of several DNA preparation protocols and describe a method that is economical and practical and reliably yields large amounts--usually 10-fold increased compared to earlier protocols--of highly pure genomic DNA for sophisticated downstream applications. This method was optimized for cultures of a variety of pathogenic and environmental mycobacterial species and proven to be suitable for direct mycobacterial DNA extraction from infected insect specimens.
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Registered trials
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