ArticleApplied and environmental microbiology2009
Discovery of stable and variable differences in the Mycobacterium avium subsp. paratuberculosis type I, II, and III genomes by pan-genome microarray analysis.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- Computational Methods in Immunoinformatics: Epitope Discovery and Diagnostic Applications.ACS omega · 2025Review
- High Molecular Diversity ofInternational journal of molecular sciences · 2025Article
- Comparative Genomics ofFrontiers in veterinary science · 2021Article
- Complete Genome Sequence of OvineMicroorganisms · 2020Article
- Article
- Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference toThe veterinary quarterly · 2019Review
- Comprehensive insights in the Mycobacterium avium subsp. paratuberculosis genome using new WGS data of sheep strain JIII-386 from Germany.Genome biology and evolution · 2015Article
- Genetic diversity of Mycobacterium avium subspecies paratuberculosis and the influence of strain type on infection and pathogenesis: a review.Veterinary research · 2015Review
- Typing of Mycobacterium avium subspecies paratuberculosis isolates from Newfoundland using fragment analysis.PloS one · 2015Article
- Disruption of Mycobacterium avium subsp. paratuberculosis-specific genes impairs in vivo fitness.BMC genomics · 2014Article
- Novel feature of Mycobacterium avium subsp. paratuberculosis, highlighted by characterization of the heparin-binding hemagglutinin adhesin.Journal of bacteriology · 2013Article
- Genomic variations associated with attenuation in Mycobacterium avium subsp. paratuberculosis vaccine strains.BMC microbiology · 2013Article
- Inter- and intra-subtype genotypic differences that differentiate Mycobacterium avium subspecies paratuberculosis strains.BMC microbiology · 2012Article
- Genome sequencing of ovine isolates of Mycobacterium avium subspecies paratuberculosis offers insights into host association.BMC genomics · 2012Article
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- Article
- Comparative genomics of two newly isolated Dehalococcoides strains and an enrichment using a genus microarray.The ISME journal · 2011Article
- Amplified fragment length polymorphism reveals specific epigenetic distinctions between Mycobacterium avium subspecies paratuberculosis isolates of various isolation types.Journal of clinical microbiology · 2011Article
- Exploring the zoonotic potential of Mycobacterium avium subspecies paratuberculosis through comparative genomics.PloS one · 2011Article
- Probing the pan-genome of Listeria monocytogenes: new insights into intraspecific niche expansion and genomic diversification.BMC genomics · 2010Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors.
Funding
Abstract
Mycobacterium avium subsp. paratuberculosis is an important animal pathogen widely disseminated in the environment that has also been associated with Crohn's disease in humans. Three M. avium subsp. paratuberculosis genomotypes are recognized, but genomic differences have not been fully described. To further investigate these potential differences, a 60-mer oligonucleotide microarray (designated the MAPAC array), based on the combined genomes of M. avium subsp. paratuberculosis (strain K-10) and Mycobacterium avium subsp. hominissuis (strain 104), was designed and validated. By use of a test panel of defined M. avium subsp. paratuberculosis strains, the MAPAC array was able to identify a set of large sequence polymorphisms (LSPs) diagnostic for each of the three major M. avium subsp. paratuberculosis types. M. avium subsp. paratuberculosis type II strains contained a smaller genomic complement than M. avium subsp. paratuberculosis type I and M. avium subsp. paratuberculosis type III genomotypes, which included a set of genomic regions also found in M. avium subsp. hominissuis 104. Specific PCRs for genes within LSPs that differentiated M. avium subsp. paratuberculosis types were devised and shown to accurately screen a panel (n = 78) of M. avium subsp. paratuberculosis strains. Analysis of insertion/deletion region INDEL12 showed deletion events causing a reduction in the complement of mycobacterial cell entry genes in M. avium subsp. paratuberculosis type II strains and significantly altering the coding of a major immunologic protein (MPT64) associated with persistence and granuloma formation. Analysis of MAPAC data also identified signal variations in several genomic regions, termed variable genomic islands (vGIs), suggestive of transient duplication/deletion events. vGIs contained significantly low GC% and were immediately flanked by insertion sequences, integrases, or short inverted repeat sequences. Quantitative PCR demonstrated that variation in vGI signals could be associated with colony growth rate and morphology.
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