ArticleApplied and environmental microbiology2009
Characterization of serine proteinase expression in Agaricus bisporus and Coprinopsis cinerea by using green fluorescent protein and the A. bisporus SPR1 promoter.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Tyrosinase Recovered from White Button Mushroom Waste: Extraction, Characterization, and Application in Casein Cross-Linking.Journal of agricultural and food chemistry · 2026Article
- Comparative Transcriptome and Endophytic Bacterial Community Analysis ofFrontiers in microbiology · 2021Article
- Article
- Potential molecular mechanisms for fruiting body formation of Cordyceps illustrated in the case ofMycology · 2017Article
- Functional analysis of Agaricus bisporus serine proteinase 1 reveals roles in utilization of humic rich substrates and adaptation to the leaf-litter ecological niche.Environmental microbiology · 2016Article
- The good, the bad and the tasty: The many roles of mushrooms.Studies in mycology · 2016Article
- Current technologies and related issues for mushroom transformation.Mycobiology · 2015Review
- High level protein-purification allows the unambiguous polypeptide determination of latent isoform PPO4 of mushroom tyrosinase.Phytochemistry · 2014Article
- Monitoring the metabolic state of fungal hyphae and the presence of melanin by nonlinear spectral imaging.Applied and environmental microbiology · 2013Article
- Transcriptome and proteome exploration to provide a resource for the study of Agrocybe aegerita.PloS one · 2013Article
- Genome sequence of the button mushroom Agaricus bisporus reveals mechanisms governing adaptation to a humic-rich ecological niche.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Transformation of Epichloë typhina by electroporation of conidia.BMC research notes · 2011Article
- Establishing molecular tools for genetic manipulation of the pleuromutilin-producing fungus Clitopilus passeckerianus.Applied and environmental microbiology · 2009Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The Agaricus bisporus serine proteinase 1 (SPR1) appears to be significant in both mycelial nutrition and senescence of the fruiting body. We report on the construction of an SPR promoter::green fluorescent protein (GFP) fusion cassette, pGreen_hph1_SPR_GFP, for the investigation of temporal and developmental expression of SPR1 in homobasidiomycetes and to determine how expression is linked to physiological and environmental stimuli. Monitoring of A. bisporus pGreen_hph1_SPR_GFP transformants on media rich in ammonia or containing different nitrogen sources demonstrated that SPR1 is produced in response to available nitrogen. In A. bisporus fruiting bodies, GFP activity was localized to the stipe of postharvest senescing sporophores. pGreen_hph1_SPR_GFP was also transformed into the model basidiomycete Coprinopsis cinerea. Endogenous C. cinerea proteinase activity was profiled during liquid culture and fruiting body development. Maximum activity was observed in the mature cap, while activity dropped during autolysis. Analysis of the C. cinerea genome revealed seven genes showing significant homology to the A. bisporus SPR1 and SPR2 genes. These genes contain the aspartic acid, histidine, and serine residues common to serine proteinases. Analysis of the promoter regions revealed at least one CreA and several AreA regulatory motifs in all sequences. Fruiting was induced in C. cinerea dikaryons, and fluorescence was determined in different developmental stages. GFP expression was observed throughout the life cycle, demonstrating that serine proteinase can be active in all stages of C. cinerea fruiting body development. Serine proteinase expression (GFP fluorescence) was most concentrated during development of young tissue, which may be indicative of high protein turnover during cell differentiation.
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