ArticleMolecular and cellular biology2009
Regulation of nuclear positioning and dynamics of the silent mating type loci by the yeast Ku70/Ku80 complex.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 70 citations in OpenAlex.
- Article
- Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III.PLoS genetics · 2023Article
- The regional sequestration of heterochromatin structural proteins is critical to form and maintain silent chromatin.Epigenetics & chromatin · 2022Review
- Micro-CT for Biological and Biomedical Studies: A Comparison of Imaging Techniques.Journal of imaging · 2021Review
- Actin and Nuclear Envelope Components Influence Ectopic Recombination in the Absence of Swr1.Genetics · 2019Article
- Mutations in the PCNA DNA Polymerase Clamp ofGenetics · 2019Article
- A Sir2-regulated locus control region in the recombination enhancer of Saccharomyces cerevisiae specifies chromosome III structure.PLoS genetics · 2019Article
- Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane.Nature communications · 2019Article
- The mRNA export adaptor Yra1 contributes to DNA double-strand break repair through its C-box domain.PloS one · 2019Article
- Chromatin and nucleosome dynamics in DNA damage and repair.Genes & development · 2017Review
- Aneuploidy as a cause of impaired chromatin silencing and mating-type specification in budding yeast.eLife · 2017Article
- Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage.Molecular biology of the cell · 2017Article
- Donor Preference Meets Heterochromatin: Moonlighting Activities of a Recombinational Enhancer in Saccharomyces cerevisiae.Genetics · 2016Article
- PolySUMOylation by Siz2 and Mms21 triggers relocation of DNA breaks to nuclear pores through the Slx5/Slx8 STUbL.Genes & development · 2016Article
- The Conformation of Yeast Chromosome III Is Mating Type Dependent and Controlled by the Recombination Enhancer.Cell reports · 2015Article
- Differential chromosome conformations as hallmarks of cellular identity revealed by mathematical polymer modeling.PLoS computational biology · 2015Article
- Regulation of recombination at yeast nuclear pores controls repair and triplet repeat stability.Genes & development · 2015Article
- Heterochromatin formation via recruitment of DNA repair proteins.Molecular biology of the cell · 2015Article
- DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells.PLoS genetics · 2014Article
- Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have examined the hypothesis that the highly selective recombination of an active mating type locus (MAT) with either HMLalpha or HMRa is facilitated by the spatial positioning of relevant sequences within the budding yeast (Saccharomyces cerevisiae) nucleus. However, both position relative to the nuclear envelope (NE) and the subnuclear mobility of fluorescently tagged MAT, HML, or HMR loci are largely identical in haploid a and alpha cells. Irrespective of mating type, the expressed MAT locus is highly mobile within the nuclear lumen, while silent loci move less and are found preferentially near the NE. The perinuclear positions of HMR and HML are strongly compromised in strains lacking the Silent information regulator, Sir4. However, HMLalpha, unlike HMRa and most telomeres, shows increased NE association in a strain lacking yeast Ku70 (yKu70). Intriguingly, we find that the yKu complex is associated with HML and HMR sequences in a mating-type-specific manner. Its abundance decreases at the HMLalpha donor locus and increases transiently at MATa following DSB induction. Our data suggest that mating-type-specific binding of yKu to HMLalpha creates a local chromatin structure competent for recombination, which cooperates with the recombination enhancer to direct donor choice for gene conversion of the MATa locus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.