ArticleEukaryotic cell2009
GAP45 phosphorylation controls assembly of the Toxoplasma myosin XIV complex.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Key Regulators of Parasite Biology Viewed Through a Post-Translational Modification Repertoire.Proteomics · 2025Review
- Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance in Toxoplasma gondii.PLoS pathogens · 2024Article
- PP1 phosphatase controls both daughter cell formation and amylopectin levels in Toxoplasma gondii.PLoS biology · 2024Article
- Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance inbioRxiv : the preprint server for biology · 2024Article
- TgMORN2, a MORN Family Protein Involved in the Regulation of Endoplasmic Reticulum Stress inInternational journal of molecular sciences · 2023Article
- Plasmodium falciparum GAP40 Plays an Essential Role in Merozoite Invasion and Gametocytogenesis.Microbiology spectrum · 2023Article
- Article
- The Modular Circuitry of Apicomplexan Cell Division Plasticity.Frontiers in cellular and infection microbiology · 2021Review
- Life cycle stages, specific organelles and invasion mechanisms ofParasitology · 2020Review
- Comparative and functional genomics of the protozoan parasite Babesia divergens highlighting the invasion and egress processes.PLoS neglected tropical diseases · 2019Article
- The dense granule protein 8 (GRA8) is a component of the sub-pellicular cytoskeleton in Toxoplasma gondii.Parasitology research · 2019Article
- A plasma membrane localized protein phosphatase in Toxoplasma gondii, PPM5C, regulates attachment to host cells.Scientific reports · 2019Article
- Review
- How does Toxoplama gondii invade host cells?The Journal of veterinary medical science · 2018Review
- Post-translational modifications as key regulators of apicomplexan biology: insights from proteome-wide studies.Molecular microbiology · 2018Review
- The serine/threonine phosphatases of apicomplexan parasites.Molecular microbiology · 2017Review
- Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog.Parasites & vectors · 2016Article
- The apicomplexan glideosome and adhesins - Structures and function.Journal of structural biology · 2015Review
- Phosphorylation of a Myosin Motor by TgCDPK3 Facilitates Rapid Initiation of Motility during Toxoplasma gondii egress.PLoS pathogens · 2015Article
- Targeting Toxoplasma tubules: tubulin, microtubules, and associated proteins in a human pathogen.Eukaryotic cell · 2015Review
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4 authors.
Funding
Abstract
Toxoplasma gondii motility is powered by the myosin XIV motor complex, which consists of the myosin XIV heavy chain (MyoA), the myosin light chain (MLC1), GAP45, and GAP50, the membrane anchor of the complex. MyoA, MLC1, and GAP45 are initially assembled into a soluble complex, which then associates with GAP50, an integral membrane protein of the parasite inner membrane complex. While all proteins in the myosin XIV motor complex are essential for parasite survival, the specific role of GAP45 remains unclear. We demonstrate here that final assembly of the motor complex is controlled by phosphorylation of GAP45. This protein is phosphorylated on multiple residues, and by using mass spectroscopy, we have identified two of these, Ser(163) and Ser(167). The importance of these phosphorylation events was determined by mutation of Ser(163) and Ser(167) to Glu and Ala residues to mimic phosphorylated and nonphosphorylated residues, respectively. Mutation of Ser(163) and Ser(167) to either Ala or Glu residues does not affect targeting of GAP45 to the inner membrane complex or its association with MyoA and MLC1. Mutation of Ser(163) and Ser(167) to Ala residues also does not affect assembly of the mutant GAP45 protein into the myosin motor complex. Mutation of Ser(163) and Ser(167) to Glu residues, however, prevents association of the MyoA-MLC1-GAP45 complex with GAP50. These observations indicate that phosphorylation of Ser(163) and Ser(167) in GAP45 controls the final step in assembly of the myosin XIV motor complex.
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