ArticleJournal of bacteriology2009
Activation of dormant bacterial genes by Nonomuraea sp. strain ATCC 39727 mutant-type RNA polymerase.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
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- Serendipity Reveals the Function and Physiological Role of a Large Family of Proteins.Journal of bacteriology · 2022Article
- Rid7C, a Member of the YjgF/YER057c/UK114 (Rid) Protein Family, Is a Novel Endoribonuclease That Regulates the Expression of a Specialist RNA Polymerase Involved in Differentiation in Nonomuraea gerenzanensis.Journal of bacteriology · 2022Article
- Interplay between Non-Coding RNA Transcription, Stringent/Relaxed Phenotype and Antibiotic Production inAntibiotics (Basel, Switzerland) · 2021Article
- Genetically engineered rpsL merodiploidy impacts secondary metabolism and antibiotic resistance in Streptomyces.World journal of microbiology & biotechnology · 2021Article
- The Application of Ribosome Engineering to Natural Product Discovery and Yield Improvement inAntibiotics (Basel, Switzerland) · 2019Review
- Ribosome engineering and fermentation optimization leads to overproduction of tiancimycin A, a new enediyne natural product from Streptomyces sp. CB03234.Journal of industrial microbiology & biotechnology · 2018Article
- Insights into microbial cryptic gene activation and strain improvement: principle, application and technical aspects.The Journal of antibiotics · 2017Review
- The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated "magic" RNA polymerase.Scientific reports · 2016Article
- Engineering microbial hosts for production of bacterial natural products.Natural product reports · 2016Review
- Characterization of representative rpoB gene mutations leading to a significant change in toyocamycin production of Streptomyces diastatochromogenes 1628.Journal of industrial microbiology & biotechnology · 2016Article
- Fitness Cost of Rifampin Resistance in Neisseria meningitidis: In Vitro Study of Mechanisms Associated with rpoB H553Y Mutation.Antimicrobial agents and chemotherapy · 2015Article
- The Sound of Silence: Activating Silent Biosynthetic Gene Clusters in Marine Microorganisms.Marine drugs · 2015Review
- The impact of drug resistance on Mycobacterium tuberculosis physiology: what can we learn from rifampicin?Emerging microbes & infections · 2014Review
- Activating the expression of bacterial cryptic genes by rpoB mutations in RNA polymerase or by rare earth elements.Journal of industrial microbiology & biotechnology · 2014Review
- The respiratory chains of four strains of the alkaliphilic Bacillus clausii.FEBS open bio · 2014Article
- Activation and products of the cryptic secondary metabolite biosynthetic gene clusters by rifampin resistance (rpoB) mutations in actinomycetes.Journal of bacteriology · 2013Article
- rRNA (rrn) operon-engineered Bacillus subtilis as a feasible test organism for antibiotic discovery.Antimicrobial agents and chemotherapy · 2013Article
- New strategies for drug discovery: activation of silent or weakly expressed microbial gene clusters.Applied microbiology and biotechnology · 2013Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is accumulating evidence that the ability of actinomycetes to produce antibiotics and other bioactive secondary metabolites has been underestimated due to the presence of cryptic gene clusters. The activation of dormant genes is therefore one of the most important areas of experimental research for the discovery of drugs in these organisms. The recent observation that several actinomycetes possess two RNA polymerase beta-chain genes (rpoB) has opened up the possibility, explored in this study, of developing a new strategy to activate dormant gene expression in bacteria. Two rpoB paralogs, rpoB(S) and rpoB(R), provide Nonomuraea sp. strain ATCC 39727 with two functionally distinct and developmentally regulated RNA polymerases. The product of rpoB(R), the expression of which increases after transition to stationary phase, is characterized by five amino acid substitutions located within or close to the so-called rifampin resistance clusters that play a key role in fundamental activities of RNA polymerase. Here, we report that rpoB(R) markedly activated antibiotic biosynthesis in the wild-type Streptomyces lividans strain 1326 and also in strain KO-421, a relaxed (rel) mutant unable to produce ppGpp. Site-directed mutagenesis demonstrated that the rpoB(R)-specific missense H426N mutation was essential for the activation of secondary metabolism. Our observations also indicated that mutant-type or duplicated, rpoB often exists in nature among rare actinomycetes and will thus provide a basis for further basic and applied research.
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