ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2009
Raising gestational choline intake alters gene expression in DMBA-evoked mammary tumors and prolongs survival.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Maternal choline supplementation during the third trimester of pregnancy improves infant information processing speed: a randomized, double-blind, controlled feeding study.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Trial
- Choline, a nexus for neurocognitive nutrients: A narrative review.Nutrition research (New York, N.Y.) · 2026Review
- Article
- Brain DNA Methylation Atlas of AppAging cell · 2025Article
- KLF9 in cancer: a potential prognostic marker and therapeutic target.Frontiers in oncology · 2025Review
- Maternal Choline Supplementation as a Potential Therapy for Down Syndrome: Assessment of Effects Throughout the Lifespan.Frontiers in aging neuroscience · 2021Article
- Article
- The Impact of Traditional Food and Lifestyle Behavior on Epigenetic Burden of Chronic Disease.Global challenges (Hoboken, NJ) · 2017Review
- Neuroprotective Actions of Dietary Choline.Nutrients · 2017Review
- Maternal Choline Supplementation: A Potential Prenatal Treatment for Down Syndrome and Alzheimer's Disease.Current Alzheimer research · 2016Article
- A 24-hour dietary recall for assessing the intake pattern of choline among Bangladeshi pregnant women at their third trimester of pregnancy.Central Asian journal of global health · 2014Article
- Neuroprotective actions of perinatal choline nutrition.Clinical chemistry and laboratory medicine · 2013Review
- Cancer control and prevention by nutrition and epigenetic approaches.Antioxidants & redox signaling · 2012Review
- Redefining the impact of nutrition on breast cancer incidence: is epigenetics involved?Nutrition research reviews · 2012Review
- Choline nutrition programs brain development via DNA and histone methylation.Central nervous system agents in medicinal chemistry · 2012Review
- Choline and betaine in health and disease.Journal of inherited metabolic disease · 2011Review
- High intakes of choline and betaine reduce breast cancer mortality in a population-based study.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Gestational choline supply regulates methylation of histone H3, expression of histone methyltransferases G9a (Kmt1c) and Suv39h1 (Kmt1a), and DNA methylation of their genes in rat fetal liver and brain.The Journal of biological chemistry · 2009Article
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Authors and funding
7 authors at 1 institution in 1 country.
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Abstract
Choline is an essential nutrient that serves as a donor of metabolic methyl groups used during gestation to establish the epigenetic DNA methylation patterns that modulate tissue-specific gene expression. Because the mammary gland begins its development prenatally, we hypothesized that choline availability in utero may affect the gland's susceptibility to cancer. During gestational days 11-17, pregnant rats were fed a control, choline-supplemented, or choline-deficient diet (8, 36, and 0 mmol/kg of choline, respectively). On postnatal day 65, the female offspring received 25 mg/kg of a carcinogen 7,12-dimethylbenz[alpha]anthracene. Approximately 70% of the rats developed mammary adenocarcinomas; prenatal diet did not affect tumor latency, incidence, size, and multiplicity. Tumor growth rate was inversely related to choline content in the prenatal diet, resulting in 50% longer survival until euthanasia, determined by tumor size, of the prenatally choline-supplemented rats compared with the prenatally choline-deficient rats. This was accompanied by distinct expression patterns of approximately 70 genes in tumors derived from the three dietary groups. Tumors from the prenatally choline-supplemented rats overexpressed genes that confer favorable prognosis in human cancers (Klf6, Klf9, Nid2, Ntn4, Per1, and Txnip) and underexpressed those associated with aggressive disease (Bcar3, Cldn12, Csf1, Jag1, Lgals3, Lypd3, Nme1, Ptges2, Ptgs1, and Smarcb1). DNA methylation within the tumor suppressor gene, stratifin (Sfn, 14-3-3sigma), was proportional to the prenatal choline supply and correlated inversely with the expression of its mRNA and protein in tumors, suggesting that an epigenetic mechanism may underlie the altered molecular phenotype and tumor growth. Our results suggest a role for adequate maternal choline nutrition during pregnancy in prevention/alleviation of breast cancer in daughters.
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