ArticleThe Journal of biological chemistry2009
Biomechanical Regulation of Endothelium-dependent Events Critical for Adaptive Remodeling.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Fluid shear stress regulates placental growth factor expression via heme oxygenase 1 and iron.Scientific reports · 2021Article
- Lectin Staining of Microvascular Glycocalyx in Microfluidic Cancer Cell Extravasation Assays.Life (Basel, Switzerland) · 2021Article
- Chemokine mediated signalling within arteries promotes vascular smooth muscle cell recruitment.Communications biology · 2020Article
- The Roles of AMPK in Revascularization.Cardiology research and practice · 2020Review
- Endothelial/Epithelial Mesenchymal Transition in Ascending Aortas of Patients With Bicuspid Aortic Valve.Frontiers in cardiovascular medicine · 2019Review
- In vivo modulation of endothelial polarization by Apelin receptor signalling.Nature communications · 2016Article
- Fluid shear stress upregulates placental growth factor in the vessel wall via NADPH oxidase 4.American journal of physiology. Heart and circulatory physiology · 2015Article
- Mechanisms of Amplified Arteriogenesis in Collateral Artery Segments Exposed to Reversed Flow Direction.Arteriosclerosis, thrombosis, and vascular biology · 2015Article
- Biomechanical regulation of vascular smooth muscle cell functions: from in vitro to in vivo understanding.Journal of the Royal Society, Interface · 2014Review
- New tools and new biology: recent miniaturized systems for molecular and cellular biology.Molecules and cells · 2013Review
- The role of shear-induced transforming growth factor-β signaling in the endothelium.Arteriosclerosis, thrombosis, and vascular biology · 2013Article
- A sensitive chemotaxis assay using a novel microfluidic device.BioMed research international · 2013Article
- Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels.Nature protocols · 2012Article
- Hot embossing for fabrication of a microfluidic 3D cell culture platform.Biomedical microdevices · 2011Article
- Krüppel-like factor 5 protects against dextran sulfate sodium-induced colonic injury in mice by promoting epithelial repair.Gastroenterology · 2011Article
- Effects of disturbed flow on vascular endothelium: pathophysiological basis and clinical perspectives.Physiological reviews · 2011Review
- A low resistance microfluidic system for the creation of stable concentration gradients in a defined 3D microenvironment.Biomedical microdevices · 2010Article
- Linking cell structure to gene regulation: signaling events and expression controls on the model genes PAI-1 and CTGF.Cellular signalling · 2010Review
- Mammalian Krüppel-like factors in health and diseases.Physiological reviews · 2010Review
- Direct-contact co-culture between smooth muscle and endothelial cells inhibits TNF-alpha-mediated endothelial cell activation.American journal of physiology. Heart and circulatory physiology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Alterations in hemodynamic shear stress acting on the vascular endothelium are critical for adaptive arterial remodeling. The molecular mechanisms regulating this process, however, remain largely uncharacterized. Here, we sought to define the responses evoked in endothelial cells exposed to shear stress waveforms characteristic of coronary collateral vessels and the subsequent paracrine effects on smooth muscle cells. A lumped parameter model of the human coronary collateral circulation was used to simulate normal and adaptive remodeling coronary collateral shear stress waveforms. These waveforms were then applied to cultured human endothelial cells (EC), and the resulting differences in EC gene expression were assessed by genome-wide transcriptional profiling to identify genes distinctly regulated by collateral flow. Analysis of these transcriptional programs identified several genes to be differentially regulated by collateral flow, including genes important for endothelium-smooth muscle interactions. In particular, the transcription factor KLF2 was up-regulated by the adaptive remodeling coronary collateral waveform, and several of its downstream targets displayed the expected modulation, including the down-regulation of connective tissue growth factor. To assess the effect of endothelial KLF2 expression on smooth muscle cell migration, a three-dimensional microfluidic assay was developed. Using this three-dimensional system, we showed that KLF2-expressing EC co-cultured with SMC significantly reduce SMC migration compared with control EC and that this reduction can be rescued by the addition of exogenous connective tissue growth factor. Collectively, these results demonstrate that collateral flow evokes distinct EC gene expression profiles and functional phenotypes that subsequently influence vascular events important for adaptive remodeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.