ArticleBMC genomics2008
Resequencing microarray probe design for typing genetically diverse viruses: human rhinoviruses and enteroviruses.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Multiplex detection and identification of viral, bacterial, and protozoan pathogens in human blood and plasma using an expanded high-density resequencing microarray platform.Frontiers in molecular biosciences · 2024Article
- Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia.Archives of virology · 2018Article
- Molecular diagnosis of microbial copathogens with influenza A(H1N1)pdm09 in Oaxaca, Mexico.Research and reports in tropical medicine · 2018Article
- A resequencing pathogen microarray method for high-throughput molecular diagnosis of multiple etiologies associated with central nervous system infection.Archives of virology · 2017Article
- DNA Sequence Signatures for Rapid Detection of Six Target Bacterial Pathogens Using PCR Assays.Microbiology insights · 2015Article
- Article
- Resequencing microarray technology for genotyping human papillomavirus in cervical smears.PloS one · 2014Article
- Pathogen chip for respiratory tract infections.Journal of clinical microbiology · 2013Article
- Molecular diagnostic and genetic characterization of highly pathogenic viruses: application during Crimean-Congo haemorrhagic fever virus outbreaks in Eastern Europe and the Middle East.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2013Article
- Article
- Leapfrog diagnostics: Demonstration of a broad spectrum pathogen identification platform in a resource-limited setting.Health research policy and systems · 2012Article
- Reconstructed ancestral sequences improve pathogen identification using resequencing DNA microarrays.PloS one · 2010Article
- Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm.BMC genomics · 2010Article
- Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses.Journal of virology · 2010Article
- VIPR: A probabilistic algorithm for analysis of microbial detection microarrays.BMC bioinformatics · 2010Article
- Broad spectrum respiratory pathogen analysis of throat swabs from military recruits reveals interference between rhinoviruses and adenoviruses.Microbial ecology · 2010Article
- Testing and validation of high density resequencing microarray for broad range biothreat agents detection.PloS one · 2009Article
- Universal detection and identification of avian influenza virus by use of resequencing microarrays.Journal of clinical microbiology · 2009Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFebrile respiratory illness (FRI) has a high impact on public health and global economics and poses a difficult challenge for differential diagnosis. A particular issue is the detection of genetically diverse pathogens, i.e. human rhinoviruses (HRV) and enteroviruses (HEV) which are frequent causes of FRI. Resequencing Pathogen Microarray technology has demonstrated potential for differential diagnosis of several respiratory pathogens simultaneously, but a high confidence design method to select probes for genetically diverse viruses is lacking.
resultsUsing HRV and HEV as test cases, we assess a general design strategy for detecting and serotyping genetically diverse viruses. A minimal number of probe sequences (26 for HRV and 13 for HEV), which were potentially capable of detecting all serotypes of HRV and HEV, were determined and implemented on the Resequencing Pathogen Microarray RPM-Flu v.30/31 (Tessarae RPM-Flu). The specificities of designed probes were validated using 34 HRV and 28 HEV strains. All strains were successfully detected and identified at least to species level. 33 HRV strains and 16 HEV strains could be further differentiated to serotype level.
conclusionThis study provides a fundamental evaluation of simultaneous detection and differential identification of genetically diverse RNA viruses with a minimal number of prototype sequences. The results demonstrated that the newly designed RPM-Flu v.30/31 can provide comprehensive and specific analysis of HRV and HEV samples which implicates that this design strategy will be applicable for other genetically diverse viruses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.