ArticleJournal of neurochemistry2009
Afamin is synthesized by cerebrovascular endothelial cells and mediates alpha-tocopherol transport across an in vitro model of the blood-brain barrier.
Article in Journal of neurochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- A proteomics perspective on 2 years of high-intensity training in horses: a pilot study.Scientific reports · 2024Article
- Which neuroimaging and fluid biomarkers method is better in theranostic of Alzheimer's disease? An umbrella review.IBRO neuroscience reports · 2024Review
- Article
- Evaluation of Afamin Level after Bariatric Surgery in Patient with Obesity.Journal of clinical medicine · 2023Article
- Comparative Proteomic Assessment of Normal vs. Polyhydramnios Amniotic Fluid Based on Computational Analysis.Biomedicines · 2022Article
- Diagnostic circulating biomarkers to detect vision-threatening diabetic retinopathy: Potential screening tool of the future?Acta ophthalmologica · 2022Review
- Bridging Size and Charge Effects of Mesoporous Silica Nanoparticles for Crossing the Blood-Brain Barrier.Frontiers in chemistry · 2022Article
- The Physiological Roles of Vitamin E and Hypovitaminosis E in the Transition Period of High-Yielding Dairy Cows.Animals : an open access journal from MDPI · 2021Review
- Serum Afamin a Novel Marker of Increased Hepatic Lipid Content.Frontiers in endocrinology · 2021Observational
- The Role of Brain Barriers in Maintaining Brain Vitamin Levels.Annual review of nutrition · 2019Review
- Plasma Biomarkers: Potent Screeners of Alzheimer's Disease.American journal of Alzheimer's disease and other dementias · 2019Review
- [LC-MS/MS-based screening of new protein biomarkers for cervical precancerous lesions and cervical cancer].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2019Article
- Assessment of Serum Concentrations of Adropin, Afamin, and Neudesin in Children with Type 1 Diabetes.BioMed research international · 2019Article
- The detection of age-, gender-, and region-specific changes in mouse brain tocopherol levels via the application of different validated HPLC methods.Neurochemical research · 2018Article
- Article
- The vitamin E-binding protein afamin increases in maternal serum during pregnancy.Clinica chimica acta; international journal of clinical chemistry · 2014Article
- Plasma protein profiling of Mild Cognitive Impairment and Alzheimer's disease using iTRAQ quantitative proteomics.Proteome science · 2014Article
- A comparative analysis of synonymous codon usage bias pattern in human albumin superfamily.TheScientificWorldJournal · 2014Article
- Analytical characterization and clinical evaluation of an enzyme-linked immunosorbent assay for measurement of afamin in human plasma.Clinica chimica acta; international journal of clinical chemistry · 2013Article
- Detection of renal tissue and urinary tract proteins in the human urine after space flight.PloS one · 2013Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Alpha-tocopherol (alphaTocH), a member of the vitamin E family, is essential for normal neurological function. Despite the importance of alphaTocH transport into the CNS, transfer mechanisms across the blood-brain barrier (BBB) are not entirely clear. We here investigate whether afamin, a known alphaTocH-binding protein, contributes to alphaTocH transport across an in vitro model of the BBB consisting of primary porcine brain capillary endothelial cells (BCEC) and basolaterally cultured astrocytoma cells. Exogenously added afamin had no adverse effects on BCEC viability or barrier function and was transported across BCEC Transwell cultures. Furthermore, alphaTocH transport across polarized BCEC cultures to astrocytoma cells is facilitated by afamin, though to a lesser extent than by high-density lipoprotein-mediated transport, an essential and in vivo operating alphaTocH import pathway at the cerebrovasculature. We also demonstrate that porcine BCEC endogenously synthesize afamin. In line with these in vitro findings, afamin was detected by immunohistochemistry in porcine, human postmortem, and mouse brain, where prominent staining was observed almost exclusively in the cerebrovasculature. The demonstration of afamin mRNA expression in isolated brain capillaries suggests that afamin might be a new family member of binding/transport proteins contributing to alphaTocH homeostasis at the BBB in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.