ArticleMolecular and cellular biology1991
RAP1 is required for BAS1/BAS2- and GCN4-dependent transcription of the yeast HIS4 gene.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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81 citing papers in PubMed, 146 citations in OpenAlex.
- Context-dependent function of the transcriptional regulator Rap1 in gene silencing and activation inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Context dependent function of the transcriptional regulator Rap1 in gene silencing and activation inbioRxiv : the preprint server for biology · 2023Article
- Distinct functions of three chromatin remodelers in activator binding and preinitiation complex assembly.PLoS genetics · 2022Article
- A systematic genome-wide account of binding sites for the model transcription factor Gcn4.Genome research · 2022Article
- Gcn4 Binding in Coding Regions Can Activate Internal and Canonical 5' Promoters in Yeast.Molecular cell · 2018Article
- Combinatorial Cis-regulation in Saccharomyces Species.G3 (Bethesda, Md.) · 2016Article
- Chromatin and transcription in yeast.Genetics · 2012Review
- Extensive role of the general regulatory factors, Abf1 and Rap1, in determining genome-wide chromatin structure in budding yeast.Nucleic acids research · 2011Article
- Article
- A dynamic Bayesian network for identifying protein-binding footprints from single molecule-based sequencing data.Bioinformatics (Oxford, England) · 2010Article
- Many sequence-specific chromatin modifying protein-binding motifs show strong positional preferences for potential regulatory regions in the Saccharomyces cerevisiae genome.Nucleic acids research · 2010Article
- Cooperative regulation of ADE3 transcription by Gcn4p and Bas1p in Saccharomyces cerevisiae.Eukaryotic cell · 2009Article
- Article
- Reconstructing a network of stress-response regulators via dynamic system modeling of gene regulation.Gene regulation and systems biology · 2008Article
- High-resolution analysis of condition-specific regulatory modules in Saccharomyces cerevisiae.Genome biology · 2008Article
- Genome-wide analysis of transcriptional dependence and probable target sites for Abf1 and Rap1 in Saccharomyces cerevisiae.Nucleic acids research · 2007Article
- Yeast TFIID serves as a coactivator for Rap1p by direct protein-protein interaction.Molecular and cellular biology · 2007Article
- Glucose signaling in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2006Review
- Global analysis of the relationship between the binding of the Bas1p transcription factor and meiosis-specific double-strand DNA breaks in Saccharomyces cerevisiae.Molecular and cellular biology · 2006Article
- Inferring transcriptional modules from ChIP-chip, motif and microarray data.Genome biology · 2006Article
21 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
Funding
Abstract
The major in vitro binding activity to the Saccharomyces cerevisiae HIS4 promoter is due to the RAP1 protein. In the absence of GCN4, BAS1, and BAS2, the RAP1 protein binds to the HIS4 promoter in vivo but cannot efficiently stimulate HIS4 transcription. RAP1, which binds adjacently to BAS2 on the HIS4 promoter, is required for BAS1/BAS2-dependent activation of HIS4 basal-level transcription. In addition, the RAP1-binding site overlaps with the single high-affinity HIS4 GCN4-binding site. Even though RAP1 and GCN4 bind competitively in vitro, RAP1 is required in vivo for (i) the normal steady-state levels of GCN4-dependent HIS4 transcription under nonstarvation conditions and (ii) the rapid increase in GCN4-dependent steady-state HIS4 mRNA levels following amino acid starvation. The presence of the RAP1-binding site in the HIS4 promoter causes a dramatic increase in the micrococcal nuclease sensitivity of two adjacent regions within HIS4 chromatin: one region contains the high-affinity GCN4-binding site, and the other region contains the BAS1- and BAS2-binding sites. These results suggest that RAP1 functions at HIS4 by increasing the accessibility of GCN4, BAS1, and BAS2 to their respective binding sites when these sites are present within chromatin.
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