Evidence map›Paper›PMID 19044890›Full record

ArticleThe Journal of chemical physics2008

Polarization-dependent fluorescence of proteins bound to nanopore-confined lipid bilayers.

R-Q Li, A Marek, Alex I Smirnov, H Grebel

Abstract read
In one paragraph

Article in The Journal of chemical physics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 10 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

R-Q LiElectronic Imaging Center at NJIT and ECE Department, NJIT, Newark, New Jersey 07102, USA.
A Marek
Alex I Smirnov
H Grebel
New Jersey Institute of Technology · USNorth Carolina State University · US

Funding

Lipid nanotube arrays for membrane protein biochipsR01GM072897 · NIGMS · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI SMIRNOV, ALEX I. · 2005 to 2008
$1.0M
NIGMS NIH HHS R01 GM072897
6 · The paper itself

Abstract

Lipid bilayers are essential structural component of biological membranes of all the living species: from viruses and bacteria to plants and humans. Biophysical and biochemical properties of such membranes are important for understanding physical mechanisms responsible for drug targeting. Binding events between proteins and the membrane may be ascertained by introducing fluorescence markers (chromophores) to the proteins. Here we describe a novel biosensing platform designed to enhance signals of these fluorescence markers. Nanoporous aluminum oxide membranes with and without gold (Au) surface coating have been employed for optical detection of bound conjugated streptavidin to biotinylated lipid bilayers-a model system that mimics protein docking to the membrane surface. Unexpectedly, it was found that fluorescence signals from such structures vary when pumped with E-polarized and H-polarized incident optical beams. The origin of the observed polarization-dependent effects and the implications for enhanced fluorescence detection in a biochip format are being discussed.

Indexed as

FluorescenceBinding SitesChemistry, PhysicalComputer SimulationLipid BilayersModels, ChemicalNanostructuresProteinsTime FactorsLipid BilayersProteins

Identifiers

PMID19044890
PMCPMC2671673
OpenAlexW2061151489

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.