ArticleThe Journal of cell biology1991
Accumulation of talin in nodes at the edge of the lamellipodium and separate incorporation into adhesion plaques at focal contacts in fibroblasts.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 116 citations in OpenAlex.
- Protein force spectroscopy using magnetic tweezers: Slow and steady wins the race?Biophysical journal · 2026Review
- Vinculin-Arp2/3 interaction inhibits branched actin assembly to control migration and proliferation.Life science alliance · 2025Article
- Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites.The Journal of biological chemistry · 2016Review
- Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture.BioImpacts : BI · 2016Article
- A RIAM/lamellipodin-talin-integrin complex forms the tip of sticky fingers that guide cell migration.Nature communications · 2015Article
- Article
- Cell fate regulation by coupling mechanical cycles to biochemical signaling pathways.Current opinion in cell biology · 2009Review
- How force might activate talin's vinculin binding sites: SMD reveals a structural mechanism.PLoS computational biology · 2008Article
- The mechanisms and dynamics of (alpha)v(beta)3 integrin clustering in living cells.The Journal of cell biology · 2005Article
- Muscle ring finger protein-1 inhibits PKC{epsilon} activation and prevents cardiomyocyte hypertrophy.The Journal of cell biology · 2004Article
- Orientational order of the lamellipodial actin network as demonstrated in living motile cells.Molecular biology of the cell · 2003Article
- Mechanism of filopodia initiation by reorganization of a dendritic network.The Journal of cell biology · 2003Article
- Active ERK/MAP kinase is targeted to newly forming cell-matrix adhesions by integrin engagement and v-Src.The EMBO journal · 2000Article
- Characterization of integrin-tetraspanin adhesion complexes: role of tetraspanins in integrin signaling.The Journal of cell biology · 1999Article
- Talin contains three similar vinculin-binding sites predicted to form an amphipathic helix.The Biochemical journal · 1999Article
- ARF1 mediates paxillin recruitment to focal adhesions and potentiates Rho-stimulated stress fiber formation in intact and permeabilized Swiss 3T3 fibroblasts.The Journal of cell biology · 1998Article
- Layilin, a novel talin-binding transmembrane protein homologous with C-type lectins, is localized in membrane ruffles.The Journal of cell biology · 1998Article
- Disruption of the talin gene compromises focal adhesion assembly in undifferentiated but not differentiated embryonic stem cells.The Journal of cell biology · 1998Article
- Talin-null cells of Dictyostelium are strongly defective in adhesion to particle and substrate surfaces and slightly impaired in cytokinesis.The Journal of cell biology · 1997Article
- Inhibition of focal adhesion kinase (FAK) signaling in focal adhesions decreases cell motility and proliferation.Molecular biology of the cell · 1996Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The focal contact forms beneath F-actin-rich ribs, or cytoplasmic precursors, present in the lamellipodia of fibroblasts. The basal part of the precursor is retained at the contact as the initial adhesion plaque. We have examined the distribution of talin in the lamellipodia and adhesion plaques of chicken embryo fibroblasts relative to the process of focal contact formation. Motility of single cells was recorded with differential interference contrast or interference reflection microscopy before fixation and fluorescent staining for talin, F-actin, and vinculin. Talin is present along the extreme edge of the lamellipodium, where it is further concentrated into a series of nodes. The nodes of talin are present at the tips of both larger and finer F-actin-rich ribs and at small structural nodes at the edge of the lamellipodium. We suggest that the talin in the nodes functions, via a cross-linking activity, in the convergence of actin filaments at the membrane during development of the ribs. Talin accumulates de novo in the adhesion plaque, independent of that at the tip of the precursor, in response to contact with the substrate. This second accumulation of talin at the focal contact starts before vinculin, consistent with a sequential binding of talin at the membrane and of vinculin to talin. The results imply that talin functions independently at two steps during formation of the focal contact: the development of the F-actin-rich precursor of the contact; and development of the contact-associated adhesion plaque, both involving organization of F-actin at the membrane.
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