ArticleInternational journal of hematology2008
Dynamics of human erythroblast enucleation.
Article in International journal of hematology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Erythroblast enucleation at a glance.Journal of cell science · 2024Review
- Differentiation of cancer stem cells into erythroblasts in the presence of CoClScientific reports · 2021Article
- The localization of α-synuclein in the process of differentiation of human erythroid cells.International journal of hematology · 2018Article
- High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing.Scientific reports · 2017Article
- Dimethyl fumarate increases fetal hemoglobin, provides heme detoxification, and corrects anemia in sickle cell disease.JCI insight · 2017Article
- Tropomodulin 1 controls erythroblast enucleation via regulation of F-actin in the enucleosome.Blood · 2017Article
- The Asymmetric Cell Division Regulators Par3, Scribble and Pins/Gpsm2 Are Not Essential for Erythroid Development or Enucleation.PloS one · 2017Article
- Nematocytes: Discovery and characterization of a novel anculeate hemocyte in Drosophila falleni and Drosophila phalerata.PloS one · 2017Article
- Iron dose-dependent differentiation and enucleation of human erythroblasts in serum-free medium.Journal of tissue engineering and regenerative medicine · 2016Article
- The sorting of blood group active proteins during enucleation.ISBT science series · 2015Article
- Red blood cell generation by three-dimensional aggregate cultivation of late erythroblasts.Tissue engineering. Part A · 2015Article
- Melanocortins contribute to sequential differentiation and enucleation of human erythroblasts via melanocortin receptors 1, 2 and 5.PloS one · 2015Article
- Monomethylfumarate induces γ-globin expression and fetal hemoglobin production in cultured human retinal pigment epithelial (RPE) and erythroid cells, and in intact retina.Investigative ophthalmology & visual science · 2014Article
- A cell-based high-throughput screen for novel chemical inducers of fetal hemoglobin for treatment of hemoglobinopathies.PloS one · 2014Article
- Studying the enucleation process, DNA breakdown and telomerase activity of the K562 cell lines during erythroid differentiation in vitro.In vitro cellular & developmental biology. Animal · 2013Article
- Survivin: a new player during erythroblast maturation.Haematologica · 2012Article
- Article
- p38α controls erythroblast enucleation and Rb signaling in stress erythropoiesis.Cell research · 2012Article
- Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How human erythroblasts enucleate remains obscure, and some investigators suspect the effect of mechanical forces on enucleation in vitro. We determined the dynamics of the enucleation process of highly purified human erythroblasts and whether enucleation can occur without external mechanical forces. Highly purified human CD34(+) cells were cultured in liquid phase with interleukin-3, stem cell factor and erythropoietin (EPO) for 7 days and the generated erythroblasts were replaced in the same medium with EPO alone. In some experiments, the enucleating cells were processed without centrifugation and pipette aspiration to avoid physical stress and were directly observed by differential interference contrast (DIC) microscopy. Enucleation initiated at day 12 and the enucleation ratio (percent of enucleated reticulocytes in total cells) reached a maximum at day 14 with a value of 63 +/- 7%. The direct observation by DIC microscopy showed 61 +/- 9% of enucleation ratio at day 14. The human erythroblasts enucleated without contact with macrophage. The time required for enucleation was 8.4 +/- 3.4 min. The enucleation rate was 1.16 +/- 0.42%/h at day 12 and then decreased with a time dependent manner. The expelled nucleus was connected to the reticulocyte through plasma membrane and associated cytoskeletal elements, and spontaneous separation of the extruded nucleus from reticulocyte was extremely rare. In conclusion, human erythroblasts enucleate in a relatively short period without contact with macrophages, but nascent reticulocytes fail to completely separate from nuclei in the absence of macrophages, unless some physical force is applied to them.
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