ArticleThe Journal of clinical investigation1991
Differential adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells in primary culture.
Article in The Journal of clinical investigation, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 82 citations in OpenAlex.
- Current concepts on Pseudomonas aeruginosa interaction with human airway epithelium.PLoS pathogens · 2023Review
- Quantification of bacterial adhesion to tissue in high-throughput kinetics.Biology methods & protocols · 2023Article
- Targeting the Central Pocket of the Pseudomonas aeruginosa Lectin LecA.Chembiochem : a European journal of chemical biology · 2022Article
- Proteomic analysis of serum samples of paracoccidioidomycosis patients with severe pulmonary sequel.PLoS neglected tropical diseases · 2021Article
- Mimicking the host and its microenvironment in vitro for studying mucosal infections by Pseudomonas aeruginosa.Pathogens and disease · 2014Review
- Review
- Gene transfer by guanidinium-cholesterol cationic lipids into airway epithelial cells in vitro and in vivo.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Asialo GM1 is a receptor for Pseudomonas aeruginosa adherence to regenerating respiratory epithelial cells.Infection and immunity · 1996Article
- Pseudomonas aeruginosa strains possess specific adhesins for laminin.Infection and immunity · 1996Article
- Effects of free and liposome-encapsulated antibiotics on adherence of Pseudomonas aeruginosa to collagen type I.Antimicrobial agents and chemotherapy · 1995Article
- Characterization of Pseudomonas aeruginosa-induced MDCK cell injury: glycosylation-defective host cells are resistant to bacterial killing.Infection and immunity · 1995Article
- Protection of human respiratory epithelium from Pseudomonas aeruginosa adherence by phosphatidylglycerol liposomes.Infection and immunity · 1994Article
- Microbiology of cystic fibrosis lung infections: themes and issues.Journal of the Royal Society of Medicine · 1993Review
- In vitro study of the bronchial mucosa during Pseudomonas aeruginosa infection.Virchows Archiv. A, Pathological anatomy and histopathology · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human nasal polyps in outgrowth culture were used to study the Pseudomonas aeruginosa adhesion to respiratory cells. By scanning electron microscopy, P. aeruginosa were seen associated with ciliated cells, but by transmission electron microscopy, bacteria were never seen at the interciliary spaces or attached along cilia, but were identified trapped at the extremities of cilia, usually as bacterial aggregates. A fibronectin-containing fibrillar material was seen associated with aggregated bacteria. By time-lapse video microscopy, bacteria were seen to aggregate in the culture medium following their addition to the culture wells. Progressively, these aggregates were trapped by cilia or attached to migrating cells of a lower cell layer that protruded beneath the upper layer cells, at the outgrowth periphery. P. aeruginosa adhesion to these lower cell layer migrating cells was significantly higher than to ciliated or nonciliated cells of the upper cell layer. Migrating cells were intensely labeled by the complexes Con A and arachis hypogea agglutinin (PNA)-FITC, in contrast to the other cells. The percentage of PNA-labeled cells with attached bacteria was significantly higher than that without bacteria. These results suggest that changes of cell surface glycoconjugates related with cell migration may favor P. aeruginosa adhesion to respiratory cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.