Evidence map›Paper›PMID 19040565›Full record

ArticleCell proliferation2008

In vivo characterization of transplanted human embryonic stem cell-derived pancreatic endocrine islet cells.

A Eshpeter, J Jiang, M Au, R V Rajotte, K Lu, J S Lebkowski, A S Majumdar, G S Korbutt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
4.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 37 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. Generation of β cells from human pluripotent stem cells: are we there yet?Annals of the New York Academy of Sciences · 2014
    Review
  8. Review
  9. Generating β cells from stem cells-the story so far.Cold Spring Harbor perspectives in medicine · 2012
    Review
  10. Article
  11. Insulin-producing surrogate β-cells from embryonic stem cells: are we there yet?Molecular therapy : the journal of the American Society of Gene Therapy · 2011
    Review
  12. Article
  13. Review
  14. Article
  15. Stem cell therapy for type 1 diabetes mellitus.Nature reviews. Endocrinology · 2010
    Review
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

A EshpeterAlberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
J JiangAlberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
M AuAlberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
R V RajotteAlberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
K LuAlberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
J S LebkowskiAlberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
A S MajumdarAlberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
G S KorbuttAlberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
Menlo School · USUniversity of Alberta · CAGeron (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesIslet-like clusters (ILCs), differentiated from human embryonic stem cells (hESCs), were characterized both before and after transplantation under the kidney capsule of streptozotocin-induced diabetic immuno-incompetent mice. MATERIALS AND

methodsMultiple independent ILC preparations (n = 8) were characterized by immunohistochemistry, flow cytometry and cell insulin content, with six preparations transplanted into diabetic mice (n = 42), compared to controls, which were transplanted with either a human fibroblast cell line or undifferentiated hESCs (n = 28).

resultsPrior to transplantation, ILCs were immunoreactive for the islet hormones insulin, C-peptide and glucagon, and for the ductal epithelial marker cytokeratin-19. ILCs also had cellular insulin contents similar to or higher than human foetal islets. Expression of islet and pancreas-specific cell markers was maintained for 70 days post-transplantation. The mean survival of recipients was increased by transplanted ILCs as compared to transplanted human fibroblast cells (P < 0.0001), or undifferentiated hESCs (P < 0.042). Graft function was confirmed by secretion of human C-peptide in response to an oral bolus of glucose.

conclusionshESC-derived ILC grafts continued to contain cells that were positive for islet endocrine hormones and were shown to be functional by their ability to secrete human C-peptide. Further enrichment and maturation of ILCs could lead to generation of a sufficient source of insulin-producing cells for transplantation into patients with type 1 diabetes.

Indexed as

Islets of Langerhans TransplantationAnimalsCell DifferentiationCell LineDNAEmbryonic Stem CellsEndocrine CellsFlow CytometryHumansInsulinIslets of LangerhansKaplan-Meier EstimateMiceMice, Inbred NODDNAInsulin

Identifiers

PMID19040565
PMCPMC6495805
OpenAlexW1985724122

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.