ArticleThe Journal of investigative dermatology2009
Mcl-1 functions as major epidermal survival protein required for proper keratinocyte differentiation.
Article in The Journal of investigative dermatology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Differential Cellular Responses to Class I and II Sphingomyelinase D: Unraveling the Mechanisms ofInternational journal of molecular sciences · 2025Article
- FTO Alleviates Hepatic Ischemia-Reperfusion Injury by Regulating Apoptosis and Autophagy.Gastroenterology research and practice · 2025Article
- Myeloid cell leukemia-1 expression in cancers of the oral cavity: a scoping review.Cancer cell international · 2022Article
- Application of polymerized porcine hemoglobin in theFrontiers in bioengineering and biotechnology · 2022Article
- Apoptotic stress-induced FGF signalling promotes non-cell autonomous resistance to cell death.Nature communications · 2021Article
- The multiple mechanisms of MCL1 in the regulation of cell fate.Communications biology · 2021Review
- High ROS Production by Celecoxib and Enhanced Sensitivity for Death Ligand-Induced Apoptosis in Cutaneous SCC Cell Lines.International journal of molecular sciences · 2021Article
- Article
- Mcl-1 and Bcl-xL are essential for survival of the developing nervous system.Cell death and differentiation · 2019Article
- Mechanism of G9a inhibitor BIX‑01294 acting on U251 glioma cells.Molecular medicine reports · 2016Article
- Regenerative and reparative effects of human chorion-derived stem cell conditioned medium on photo-aged epidermal cells.Cell cycle (Georgetown, Tex.) · 2016Article
- Neuroprotective effect of Shenqi Fuzheng injection pretreatment in aged rats with cerebral ischemia/reperfusion injury.Neural regeneration research · 2016Article
- Alpinetin inhibits lung cancer progression and elevates sensitization drug-resistant lung cancer cells to cis-diammined dichloridoplatium.Drug design, development and therapy · 2015Article
- Epidermal expression of neuropilin 1 protects murine keratinocytes from UVB-induced apoptosis.PloS one · 2012Article
- MCL1 increases primitive thymocyte viability in female mice and promotes thymic expansion into adulthood.International immunology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Rapid downregulation of the antiapoptotic Bcl-2 family protein myeloid cell leukemia 1 (Mcl-1) is required for UV-induced apoptosis, underlining an important role for Mcl-1 in epidermal pathology. To determine if Mcl-1 has a specific role in normal keratinocyte (KC) biology, Mcl-1 was downregulated in human KCs by RNAi and these KCs were induced to differentiate in organotypic raft cultures. Mcl-1 shRNA organotypic cultures showed increased levels of spontaneous premature apoptosis, implicating Mcl-1 as an essential KC survival protein. Mcl-1-downregulated cultures also had reduced granular and cornified layers, and produced lower levels of cross-linked protein and cornified envelopes. Cornification could only partially be rescued with the general caspase inhibitor z-VAD, suggesting that reduced cornification was not entirely because of premature apoptosis. Differentiation markers (K1, K10, filaggrin, loricrin, cleaved caspase-14) were normally expressed in control organotypic cultures, but were expressed at reduced levels in organotypic cultures with downregulated Mcl-1. The defect in differentiation marker expression was independent of apoptosis as it could not be rescued by z-VAD. Thus, Mcl-1 serves two important, independent functions in epidermal KCs: acting as a major survival protein by inhibiting premature apoptosis in the spinous and granular layers to promote conification, and promoting the robust induction of KC differentiation markers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.