ArticleMolecular biology of the cell2009
Early stages of Golgi vesicle and tubule formation require diacylglycerol.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 79 citations in OpenAlex.
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- Insights into phosphatidic acid phosphatase and its potential role as a therapeutic target.Advances in biological regulation · 2025Review
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- Regulation of yeast polarized exocytosis by phosphoinositide lipids.Cellular and molecular life sciences : CMLS · 2024Review
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- Changes in the content of pollen total lipid and TAG inAoB PLANTS · 2023Article
- Insulin granule biogenesis and exocytosis.Cellular and molecular life sciences : CMLS · 2021Review
- Biophysical parameters of the Sec14 phospholipid exchange cycle - Effect of lipid packing in membranes.Biochimica et biophysica acta. Biomembranes · 2021Article
- Excess diacylglycerol at the endoplasmic reticulum disrupts endomembrane homeostasis and autophagy.BMC biology · 2020Article
- Mutant p53 induces Golgi tubulo-vesiculation driving a prometastatic secretome.Nature communications · 2020Article
- Defining the subcellular distribution and metabolic channeling of phosphatidylinositol.The Journal of cell biology · 2020Article
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- Lipid-dependent coupling of secretory cargo sorting and trafficking at the trans-Golgi network.FEBS letters · 2019Review
- Extracellular matrix mechanical cues regulate lipid metabolism through Lipin-1 and SREBP.Nature cell biology · 2019Article
- Biophysical Parameters of the Sec14 Phospholipid Exchange Cycle.Biophysical journal · 2019Article
- The Arabidopsis thaliana non-specific phospholipase C2 is involved in the response to Pseudomonas syringae attack.Annals of botany · 2018Article
- PLCζ Induced CaFrontiers in cell and developmental biology · 2018Article
- Cholesterol-Independent SREBP-1 Maturation Is Linked to ARF1 Inactivation.Cell reports · 2016Article
- Lipin-1 regulates autophagy clearance and intersects with statin drug effects in skeletal muscle.Cell metabolism · 2014Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the role for diacylglycerol (DAG) in membrane bud formation in the Golgi apparatus. Addition of propranolol to specifically inhibit phosphatidate phosphohydrolase (PAP), an enzyme responsible for converting phosphatidic acid into DAG, effectively prevents formation of membrane buds. The effect of PAP inhibition on Golgi membranes is rapid and occurs within 3 min. Removal of the PAP inhibitor then results in a rapid burst of buds, vesicles, and tubules that peaks within 2 min. The inability to form buds in the presence of propranolol does not appear to be correlated with a loss of ARFGAP1 from Golgi membranes, as knockdown of ARFGAP1 by RNA interference has little or no effect on actual bud formation. Rather, knockdown of ARFGAP1 results in an increase in membrane buds and a decrease of vesicles and tubules suggesting it functions in the late stages of scission. How DAG promotes bud formation is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.