ArticleMolecular biology of the cell2009
Dictyostelium Dock180-related RacGEFs regulate the actin cytoskeleton during cell motility.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- An endogenous chemorepellent directs cell movement by inhibiting pseudopods at one side of cells.Molecular biology of the cell · 2019Article
- Insight from the maximal activation of the signal transduction excitable network inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- The novel RacE-binding protein GflB sharpens Ras activity at the leading edge of migrating cells.Molecular biology of the cell · 2016Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale.Nature communications · 2014Article
- Daydreamer, a Ras effector and GSK-3 substrate, is important for directional sensing and cell motility.Molecular biology of the cell · 2013Article
- ELMO domains, evolutionary and functional characterization of a novel GTPase-activating protein (GAP) domain for Arf protein family GTPases.The Journal of biological chemistry · 2012Article
- A Gβγ effector, ElmoE, transduces GPCR signaling to the actin network during chemotaxis.Developmental cell · 2012Article
- Article
- A Ras signaling complex controls the RasC-TORC2 pathway and directed cell migration.Developmental cell · 2010Article
- Structural basis of membrane targeting by the Dock180 family of Rho family guanine exchange factors (Rho-GEFs).The Journal of biological chemistry · 2010Article
- Stochastic Dynamics of Membrane Protrusion Mediated by the DOCK180/Rac Pathway in Migrating Cells.Cellular and molecular bioengineering · 2010Article
- The role of Crk/Dock180/Rac1 pathway in the malignant behavior of human ovarian cancer cell SKOV3.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2010Article
- Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarity.Annual review of biophysics · 2010Review
- Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Cell motility of amoeboid cells is mediated by localized F-actin polymerization that drives the extension of membrane protrusions to promote forward movements. We show that deletion of either of two members of the Dictyostelium Dock180 family of RacGEFs, DockA and DockD, causes decreased speed of chemotaxing cells. The phenotype is enhanced in the double mutant and expression of DockA or DockD complements the reduced speed of randomly moving DockD null cells' phenotype, suggesting that DockA and DockD are likely to act redundantly and to have similar functions in regulating cell movement. In this regard, we find that overexpressing DockD causes increased cell speed by enhancing F-actin polymerization at the sites of pseudopod extension. DockD localizes to the cell cortex upon chemoattractant stimulation and at the leading edge of migrating cells and this localization is dependent on PI3K activity, suggesting that DockD might be part of the pathway that links PtdIns(3,4,5)P(3) production to F-actin polymerization. Using a proteomic approach, we found that DdELMO1 is associated with DockD and that Rac1A and RacC are possible in vivo DockD substrates. In conclusion, our work provides a further understanding of how cell motility is controlled and provides evidence that the molecular mechanism underlying Dock180-related protein function is evolutionarily conserved.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.