ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2008
Serotonergic transcription of human FEV reveals direct GATA factor interactions and fate of Pet-1-deficient serotonin neuron precursors.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Article
- Reorganization of postmitotic neuronal chromatin accessibility for maturation of serotonergic identity.eLife · 2022Article
- Associations between polymorphisms of SLC22A7, NGFR, ARNTL and PPP2R2B genes and Milk production traits in Chinese Holstein.BMC genomic data · 2021Article
- Generation of caudal-type serotonin neurons and hindbrain-fate organoids from hPSCs.Stem cell reports · 2021Article
- Article
- Serotonin neuron development: shaping molecular and structural identities.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- Regulatory Mechanisms Controlling Maturation of Serotonin Neuron Identity and Function.Frontiers in cellular neuroscience · 2017Article
- Divergent Roles of Central Serotonin in Adult Hippocampal Neurogenesis.Frontiers in cellular neuroscience · 2017Review
- Pet-1 Switches Transcriptional Targets Postnatally to Regulate Maturation of Serotonin Neuron Excitability.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Generation of functional human serotonergic neurons from fibroblasts.Molecular psychiatry · 2016Article
- Pet-1 Controls Tetrahydrobiopterin Pathway and Slc22a3 Transporter Genes in Serotonin Neurons.ACS chemical neuroscience · 2015Article
- High fat diet decreases beneficial effects of estrogen on serotonin-related gene expression in marmosets.Progress in neuro-psychopharmacology & biological psychiatry · 2015Article
- RNASeq-derived transcriptome comparisons reveal neuromodulatory deficiency in the CO₂ insensitive brown Norway rat.The Journal of physiology · 2015Article
- Maintenance of postmitotic neuronal cell identity.Nature neuroscience · 2014Review
- Engrailed is required in maturing serotonin neurons to regulate the cytoarchitecture and survival of the dorsal raphe nucleus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Serotonergic transcriptional networks and potential importance to mental health.Nature neuroscience · 2012Review
- Molecular genetics of mouse serotonin neurons across the lifespan.Neuroscience · 2011Review
- Long-term ovariectomy decreases serotonin neuron number and gene expression in free ranging macaques.Neuroscience · 2011Article
- Pet-1 is required across different stages of life to regulate serotonergic function.Nature neuroscience · 2010Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Altered expression of the human FEV (fifth Ewing variant) ETS transcription factor gene impacts the level of CNS serotonin (5-HT) neuron gene expression and maternal nurturing. However, the regulatory mechanisms that determine FEV expression are poorly understood. Here, we investigated the cis-regulatory control of FEV to begin to identify the upstream transcription factors that restrict FEV expression to 5-HT neurons. We find that sequences extending only 275 bp upstream of the FEV 5' untranslated region are sufficient to direct FEV transgene expression to embryonic 5-HT neurons, although sequences farther upstream are required for maintenance in adult 5-HT neurons. Two highly conserved consensus GATA factor binding sites within the 275 bp region interact with GATA factors in vitro. Chromatin immunoprecipitations with embryonic hindbrain demonstrated Gata-2 interactions with the orthologous mouse Pet-1 ETS cis-regulatory region. Mutagenesis of GATA sites revealed that one or the other site is required for serotonergic FEV transgene expression. Unexpectedly, FEV-LacZ transgenes enabled determination of 5-HT neuron precursor fate in the adult Pet-1(-/-) dorsal and median raphe nuclei and thus provided additional insight into FEV/Pet-1 function. Comparable numbers of FEV-LacZ-positive cells were detected in Pet-1(+/-) and Pet-1(-/-) adult dorsal raphe nuclei, indicating that the majority of mutant serotonergic precursors are not fated to apoptosis. However, B7 dorsal raphe cells were aberrantly distributed, suggesting a role for FEV/Pet-1 in their midline organization. Our findings identify a direct transcriptional interaction between Gata-2 and FEV and a unique marker for new insight into FEV/Pet-1 function in 5-HT neuron development.
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Registered trials
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