ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2008
Glutamatergic neuronal differentiation of mouse embryonic stem cells after transient expression of neurogenin 1 and treatment with BDNF and GDNF: in vitro and in vivo studies.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 123 citations in OpenAlex.
- Magnetic graphene oxide functionalized composite nanofibrous stem cell-based neural scaffolds.Journal of nanobiotechnology · 2026Article
- The Reconstruction of Peripheral Auditory Circuit: Recent Advances and Future Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Towards Regenerative Audiology: Immune Modulation of Adipose-Derived Mesenchymal Cells Preconditioned with Citric Acid-Coated Antioxidant-Functionalized Magnetic Nanoparticles.Medicina (Kaunas, Lithuania) · 2023Article
- Overexpression of Neurogenin 1 Negatively Regulates Osteoclast and Osteoblast Differentiation.International journal of molecular sciences · 2022Article
- A perspective on human cell models for POLG-spectrum disorders: advantages and disadvantages of CRISPR-Cas-based vs. patient-derived iPSC models.Medizinische Genetik : Mitteilungsblatt des Berufsverbandes Medizinische Genetik e.V · 2021Article
- Regulation of Spiral Ganglion Neuron Regeneration as a Therapeutic Strategy in Sensorineural Hearing Loss.Frontiers in molecular neuroscience · 2021Review
- Mechanism and Prevention of Spiral Ganglion Neuron Degeneration in the Cochlea.Frontiers in cellular neuroscience · 2021Review
- CRISPR-Mediated Knockout of Long 3' UTR mRNA Isoforms in mESC-Derived Neurons.Frontiers in genetics · 2021Review
- Stem cell-based approaches: Possible route to hearing restoration?World journal of stem cells · 2020Review
- Progress in Modeling and Targeting Inner Ear Disorders with Pluripotent Stem Cells.Stem cell reports · 2020Review
- Upregulation of Neuroprogenitor and Neural Markers via Enforced miR-124 and Growth Factor Treatment.International journal of molecular and cellular medicine · 2020Article
- Increased levels of miR-124 in human dental pulp stem cells alter the expression of neural markers.Journal of otology · 2019Article
- New molecular therapies for the treatment of hearing loss.Pharmacology & therapeutics · 2019Review
- Identification of Neural Stem Cells from Postnatal Mouse Auditory Cortex In Vitro.Stem cells and development · 2019Article
- The Presence of Neural Stem Cells and Changes in Stem Cell-Like Activity With Age in Mouse Spiral Ganglion Cells In Vivo and In Vitro.Clinical and experimental otorhinolaryngology · 2018Article
- Embryonic Stem Cell-Derived Peripheral Auditory Neurons Form Neural Connections with Mouse Central Auditory Neurons In Vitro via the α2δ1 Receptor.Stem cell reports · 2018Article
- Transcription factor induced conversion of human fibroblasts towards the hair cell lineage.PloS one · 2018Article
- Stimulation of synapse formation between stem cell-derived neurons and native brainstem auditory neurons.Scientific reports · 2017Article
- Concomitant differentiation of a population of mouse embryonic stem cells into neuron-like cells and schwann cell-like cells in a slow-flow microfluidic device.Developmental dynamics : an official publication of the American Association of Anatomists · 2017Article
- Generation of Otic Sensory Neurons from Mouse Embryonic Stem Cells in 3D Culture.Frontiers in cellular neuroscience · 2017Article
1 more citing papers are in PubMed but not listed here.
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8 authors at 1 institution in 1 country.
Funding
Abstract
Differentiation of the pluripotent neuroepithelium into neurons and glia is accomplished by the interaction of growth factors and cell-type restricted transcription factors. One approach to obtaining a particular neuronal phenotype is by recapitulating the expression of these factors in embryonic stem (ES) cells. Toward the eventual goal of auditory nerve replacement, the aim of the current investigation was to generate auditory nerve-like glutamatergic neurons from ES cells. Transient expression of Neurog1 promoted widespread neuronal differentiation in vitro; when supplemented with brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF), 75% of ES cell-derived neurons attained a glutamatergic phenotype after 5 d in vitro. Mouse ES cells were also placed into deafened guinea pig cochleae and Neurog1 expression was induced for 48 h followed by 26 d of BDNF/GDNF infusion. In vivo differentiation resulted in 50-75% of ES cells bearing markers of early neurons, and a majority of these cells had a glutamatergic phenotype. This is the first study to report a high percentage of ES cell differentiation into a glutamatergic phenotype and sets the stage for cell replacement of auditory nerve.
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