ArticleJournal of clinical microbiology2009
Classification of fowl adenovirus serotypes by use of high-resolution melting-curve analysis of the hexon gene region.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 145 citations in OpenAlex.
- Development and diagnostic validation of a recombinant Hexon L1-based indirect enzyme-linked immunosorbent assay for detecting antibodies to fowl adenovirus serotype 8b in chickens.Poultry science · 2026Article
- Trivalent fowl adenovirus vaccine (serotype-4/8b/11) provides efficient protection with long duration of immunity.Journal of veterinary science · 2026Article
- Development of recombinant fowl adenovirus serotype 4 harboring the HiBiT-Tag reporter and its utility in antiviral research.Microbiology spectrum · 2026Article
- Molecular characterization and tissue tropism of an Iraqi field isolate of fowl adenovirus serotype 8a in broiler chickens.PloS one · 2026Article
- Temporal dynamics of chicken host's molecular response against Fowl adenovirus serotype 8b infection via RNA-sequencing.BMC genomics · 2025Article
- Fowl Adenovirus Serotype 1: From Gizzard Erosion to Comprehensive Insights into Genome Organization, Epidemiology, Pathogenesis, Diagnosis, and Prevention.Veterinary sciences · 2025Review
- Detection and molecular characterisation of fowl adenovirus associated with inclusion body hepatitis and hydropericardium syndrome in Uganda.Tropical animal health and production · 2025Article
- Development of a Multiplex Conventional PCR Assay for Concurrent Detection of FAdV-4, FAdV-8b, and FAdV-11.Veterinary sciences · 2025Article
- Genomic Mosaicism in Fowl Adenovirus 3 Strains.Animals : an open access journal from MDPI · 2025Article
- A snapshot on molecular technologies for diagnosing FAdV infections.Frontiers in veterinary science · 2025Review
- Evaluation of clinical, analytical, and genotyping performance of Hex L1 real-time PCR coupled with high-resolution melting curve analysis for fowl adenovirus outbreak investigation in Morocco.Frontiers in veterinary science · 2025Article
- Molecular and histopathological characterization of fowl adenovirus serotype 4 in broiler chickens in Kashan, Iran: implications for disease control and prevention in the poultry industry.Veterinary research forum : an international quarterly journal · 2025Article
- The Knob Domain of the Fiber-1 Protein Affects the Replication of Fowl Adenovirus Serotype 4.Microorganisms · 2024Article
- Molecular characterisation of fowl adenovirus associated with hydropericardium hepatitis syndrome in broiler and layer breeders in Azerbaijan.BMC veterinary research · 2024Article
- Review
- Article
- Comparison of Penton and Hexon Genes of Fowl Adenovirus Serotype 11 in Molecular Detection of IBH in Broilers.Archives of Razi Institute · 2023Article
- Article
- Fowl adenovirus serotype 4 enters leghorn male hepatocellular cells via the clathrin-mediated endocytosis pathway.Veterinary research · 2023Article
- An efficient double-fluorescence approach for generatingFrontiers in microbiology · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of fowl adenovirus (FAdV) serotypes is of importance in epidemiological studies of disease outbreaks and the adoption of vaccination strategies. In this study, real-time PCR and subsequent high-resolution melting (HRM)-curve analysis of three regions of the hexon gene were developed and assessed for their potential in differentiating 12 FAdV reference serotypes. The results were compared to previously described PCR and restriction enzyme analyses of the hexon gene. Both HRM-curve analysis of a 191-bp region of the hexon gene and restriction enzyme analysis failed to distinguish a number of serotypes used in this study. In addition, PCR of the region spanning nucleotides (nt) 144 to 1040 failed to amplify FAdV-5 in sufficient quantities for further analysis. However, HRM-curve analysis of the region spanning nt 301 to 890 proved a sensitive and specific method of differentiating all 12 serotypes. All melt curves were highly reproducible, and replicates of each serotype were correctly genotyped with a mean confidence value of more than 99% using normalized HRM curves. Sequencing analysis revealed that each profile was related to a unique sequence, with some sequences sharing greater than 94% identity. Melting-curve profiles were found to be related mainly to GC composition and distribution throughout the amplicons, regardless of sequence identity. The results presented in this study show that the closed-tube method of PCR and HRM-curve analysis provides an accurate, rapid, and robust genotyping technique for the identification of FAdV serotypes and can be used as a model for developing genotyping techniques for other pathogens.
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