ArticlePlant physiology2009
GS52 ecto-apyrase plays a critical role during soybean nodulation.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 82 citations in OpenAlex.
- Apyrase in horticultural crops: insights into growth, stress adaptation and quality regulation.Molecular horticulture · 2025Review
- Genome-wide identification and analyses ofMolecular breeding : new strategies in plant improvement · 2024Article
- Growth regulation by apyrases: Insights from altering their expression level in different organisms.Plant physiology · 2024Review
- Article
- Activation of the plant mevalonate pathway by extracellular ATP.Nature communications · 2022Article
- Phosphate-deprivation and damage signalling by extracellular ATP.Frontiers in plant science · 2022Article
- Recent Advances Clarifying the Structure and Function of Plant Apyrases (Nucleoside Triphosphate Diphosphohydrolases).International journal of molecular sciences · 2021Review
- DORN1/P2K1 and purino-calcium signalling in plants: making waves with extracellular ATP.Annals of botany · 2020Article
- A convenient, soil-free method for the production of root nodules in soybean to study the effects of exogenous additives.Plant direct · 2019Article
- Role of CaInternational journal of molecular sciences · 2018Review
- Phosphorylation-Dependent Regulation of G-Protein Cycle during Nodule Formation in Soybean.The Plant cell · 2015Article
- Light- and temperature-regulated BjAPY2 may have a role in stem expansion of Brassica juncea.Functional & integrative genomics · 2015Article
- A purple acid phosphatase plays a role in nodule formation and nitrogen fixation in Astragalus sinicus.Plant molecular biology · 2015Article
- Extracellular ATP acts as a damage-associated molecular pattern (DAMP) signal in plants.Frontiers in plant science · 2014Review
- Specific subunits of heterotrimeric G proteins play important roles during nodulation in soybean.Plant physiology · 2013Article
- Rhizobial and mycorrhizal symbioses in Lotus japonicus require lectin nucleotide phosphohydrolase, which acts upstream of calcium signaling.Plant physiology · 2013Article
- Soybean genetic transformation: A valuable tool for the functional study of genes and the production of agronomically improved plants.Genetics and molecular biology · 2012Article
- 14-3-3 proteins SGF14c and SGF14l play critical roles during soybean nodulation.Plant physiology · 2012Article
- The Arabidopsis apyrase AtAPY1 is localized in the Golgi instead of the extracellular space.BMC plant biology · 2012Article
- RNA silencing as a tool to uncover gene function and engineer novel traits in soybean.Breeding science · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apyrases are non-energy-coupled nucleotide phosphohydrolases that hydrolyze nucleoside triphosphates and nucleoside diphosphates to nucleoside monophosphates and orthophosphates. GS52, a soybean (Glycine soja) ecto-apyrase, was previously shown to be induced very early in response to inoculation with the symbiotic bacterium Bradyrhizobium japonicum. Overexpression of the GS52 ecto-apyrase in Lotus japonicus increased the level of rhizobial infection and enhanced nodulation. These data suggest a critical role for the GS52 ecto-apyrase during nodulation. To further investigate the role of GS52 during nodulation, we used RNA interference to silence GS52 expression in soybean (Glycine max) roots using Agrobacterium rhizogenes-mediated root transformation. Transcript levels of GS52 were significantly reduced in GS52 silenced roots and these roots exhibited reduced numbers of mature nodules. Development of the nodule primordium and subsequent nodule maturation was significantly suppressed in GS52 silenced roots. Transmission electron micrographs of GS52 silenced root nodules showed that early senescence and infected cortical cells were devoid of symbiosome-containing bacteroids. Application of exogenous adenosine diphosphate to silenced GS52 roots restored nodule development. Restored nodules contained bacteroids, thus indicating that extracellular adenosine diphosphate is important during nodulation. These results clearly suggest that GS52 ecto-apyrase catalytic activity is critical for the early B. japonicum infection process, initiation of nodule primordium development, and subsequent nodule organogenesis in soybean.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.