Evidence map›Paper›PMID 19036562›Full record

ArticleNutrition (Burbank, Los Angeles County, Calif.)2009

Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro.

Qixuan Chen, Raylene A Reimer

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nutrition (Burbank, Los Angeles County, Calif.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02697305 (Effect of Intravenously and Orally Administered Branched Chain Amino Acids on Incretin Response), which is not on this map. Cited by 56 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed, 4 pooled it
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02697305 nacompletednot on this mapstarted 2014, after this paper: background citation

Effect of Intravenously and Orally Administered Branched Chain Amino Acids on Incretin Response

TypeinterventionalSponsorCharles University, Czech RepublicRan2014 to 2015Enrolled18ConditionsGlucose Metabolism DisordersArmsIV BCAA, ORAL BCAA, ORAL Placebo
3 · Its place in the literature

Who cites it

56 citing papers in PubMed, 4 syntheses or guidelines pooled it, 155 citations in OpenAlex.

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  18. Circulating Branched Chain Amino Acids and Cardiometabolic Disease.Journal of the American Heart Association · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Qixuan ChenFaculty of Kinesiology and Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
Raylene A Reimer
University of Calgary · CA

Funding

CIHR 86460-1
6 · The paper itself

Abstract

objectiveA growing body of evidence supports an antiobesity effect of dairy products; however, the mechanisms remain unclear. The objective of this study was to explore possible intestinal mechanisms by which dairy delivers an antiobesity effect. The human intestinal cell line, NCI-H716, was used to test the hypothesis that branched-chain amino acids and dairy proteins regulate satiety hormone secretion and modulate genes involved in fatty acid and cholesterol metabolism.

methodsIn dose-response (0.5%, 1.0%, 2.0%, and 3.0%) studies, the effect of leucine, isoleucine, valine, skim milk, casein, and whey on glucagon-like peptide-1 release and the expression of selected genes were tested.

resultsLeucine, isoleucine, skim milk, and casein stimulated glucagon-like peptide-1 release (P < 0.05). Isoleucine and whey downregulated the expression of intestinal-type fatty acid binding protein (i-FABP), fatty acid transport protein 4 (FATP4), Niemann-Pick C-1-like-1 protein (NPC1L1), acetyl-coenzyme A carboxylase (ACC), fatty acid synthase (FAS), sterol regulatory element-binding protein-2 (SREBP-2), and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR; P < 0.05). Leucine and valine downregulated the expression of NPC1L1, ACC, FAS, SREBP-2, and HMGCR (P < 0.05). Casein downregulated the expression of i-FABP, FATP4, ACC, FAS, SREBP-2, and HMGCR (P < 0.05). Skim milk downregulated the expression of ACC, FAS, and SREBP-2, but not i-FABP, FATP4, and NPC1L1.

conclusionThis work suggests that the antiobesity effect of dairy may be mediated, at least in part, by integration of events that promote glucagon-like peptide-1 secretion and inhibit expression of genes involved in intestinal fatty acid and cholesterol absorption and synthesis.

Indexed as

AnimalsCaseinsCell LineDietary ProteinsDose-Response Relationship, DrugFatty Acid Transport ProteinsGene Expression RegulationGlucagon-Like Peptide 1HumansIntestinal AbsorptionIsoleucineLeucineLipid MetabolismMilkMilk ProteinsRNA, MessengerCaseinsDietary ProteinsFatty Acid Transport ProteinsGlucagon-Like Peptide 1IsoleucineLeucineMilk ProteinsRNA, MessengerWhey Proteins

Identifiers

PMID19036562
PMCPMC3827015
OpenAlexW1964031110

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.