ArticleBMC cancer2008
Gene expression profile of cervical and skin tissues from human papillomavirus type 16 E6 transgenic mice.
Article in BMC cancer, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- The Clinical Significance and Immunization of MSMO1 in Cervical Squamous Cell Carcinoma Based on Bioinformatics Analysis.Frontiers in genetics · 2021Article
- Effects of HPV16 E6 protein on Daxx-induced apoptosis in C33A cells.Cellular & molecular biology letters · 2020Article
- Effect of Productive Human Papillomavirus 16 Infection on Global Gene Expression in Cervical Epithelium.Journal of virology · 2018Article
- Human papillomavirus oncogenes reprogram the cervical cancer microenvironment independently of and synergistically with estrogen.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The PDZ-Binding Motif of HPV16-E6 Oncoprotein Modulates the Keratinization and Stemness Transcriptional ProfileBioMed research international · 2017Article
- Papillomavirus E6 oncoproteins.Virology · 2013Review
- Research resource: the Endometrium Database Resource (EDR).Molecular endocrinology (Baltimore, Md.) · 2013Article
- Differential gene expression between skin and cervix induced by the E7 oncoprotein in a transgenic mouse model.Virology · 2012Article
- The E6 oncoprotein from HPV16 enhances the canonical Wnt/β-catenin pathway in skin epidermis in vivo.Molecular cancer research : MCR · 2012Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundAlthough K14E6 transgenic mice develop spontaneous tumors of the skin epithelium, no spontaneous reproductive tract malignancies arise, unless the transgenic mice were treated chronically with 17beta-estradiol. These findings suggest that E6 performs critical functions in normal adult cervix and skin, highlighting the need to define E6-controlled transcriptional programs in these tissues.
methodsWe evaluated the expression profile of 14,000 genes in skin or cervix from young K14E6 transgenic mice compared with nontransgenic. To identify differentially expressed genes a linear model was implemented using R and the LIMMA package. Two criteria were used to select the set of relevant genes. First a set of genes with a Log-odds > or = 3 were selected. Then, a hierarchical search of genes was based on Log Fold Changes.
resultsMicroarray analysis identified a total of 676 and 1154 genes that were significantly up and down-regulated, respectively, in skin from K14E6 transgenic mice. On the other hand, in the cervix from K14E6 transgenic mice we found that only 97 and 252 genes were significantly up and down-regulated, respectively. One of the most affected processes in the skin from K14E6 transgenic mice was the cell cycle. We also found that skin from transgenic mice showed down-regulation of pro-apoptotic genes and genes related to the immune response. In the cervix of K14E6 transgenic mice, we could not find affected any gene related to the cell cycle and apoptosis pathways but did observe alterations in the expression of immune response genes. Pathways such as angiogenesis, cell junction and epidermis development, also were altered in their gene expression profiles in both tissues.
conclusionExpression of the HPV16 E6 oncoprotein in our model alters expression of genes that fell into several functional groups providing insights into pathways by which E6 deregulate cell cycle progression, apoptosis, the host resistance to infection and immune function, providing new opportunities for early diagnostic markers and therapeutic drug targets.
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