ArticleProceedings of the National Academy of Sciences of the United States of America1991
Ferritin mRNA: interactions of iron regulatory element with translational regulator protein P-90 and the effect on base-paired flanking regions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 95 citations in OpenAlex.
- Sequence alignment of the primate lineage reveals evolutionary divergence and conserved secondary structural motifs in noncoding RNAs.bioRxiv : the preprint server for biology · 2026Article
- Small-Molecule Targeting of the Iron-Responsive Element in the APP mRNA 5'-UTR to Control Amyloid Translation in Alzheimer's Disease.International journal of molecular sciences · 2026Review
- Iron-dependent post transcriptional control of mitochondrial aconitase expression.Metallomics : integrated biometal science · 2023Article
- Rapid kinetics of iron responsive element (IRE) RNA/iron regulatory protein 1 and IRE-RNA/eIF4F complexes respond differently to metal ions.Nucleic acids research · 2014Article
- Mammalian iron metabolism and its control by iron regulatory proteins.Biochimica et biophysica acta · 2012Review
- Fe2+ binds iron responsive element-RNA, selectively changing protein-binding affinities and regulating mRNA repression and activation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Iron responsive mRNAs: a family of Fe2+ sensitive riboregulators.Accounts of chemical research · 2011Article
- Multiple determinants within iron-responsive elements dictate iron regulatory protein binding and regulatory hierarchy.RNA (New York, N.Y.) · 2010Article
- Direct Fe2+ sensing by iron-responsive messenger RNA:repressor complexes weakens binding.The Journal of biological chemistry · 2009Article
- Living with iron (and oxygen): questions and answers about iron homeostasis.Chemical reviews · 2009Review
- Molecular control of vertebrate iron homeostasis by iron regulatory proteins.Biochimica et biophysica acta · 2006Review
- The up-regulation of ferritin expression using a small-molecule ligand to the native mRNA.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The Brome mosaic virus subgenomic promoter hairpin is structurally similar to the iron-responsive element and functionally equivalent to the minus-strand core promoter stem-loop C.RNA (New York, N.Y.) · 2002Article
- Translation of a testis-specific Cu/Zn superoxide dismutase (SOD-1) mRNA is regulated by a 65-kilodalton protein which binds to its 5' untranslated region.Molecular and cellular biology · 1996Article
- The human immunodeficiency virus type 1 5' packaging signal structure affects translation but does not function as an internal ribosome entry site structure.Journal of virology · 1996Article
- Role of RNA secondary structure of the iron-responsive element in translational regulation of ferritin synthesis.Nucleic acids research · 1995Article
- Human ribosomal protein L7 inhibits cell-free translation in reticulocyte lysates and affects the expression of nuclear proteins upon stable transfection into Jurkat T-lymphoma cells.Nucleic acids research · 1995Article
- The importance of a single G in the hairpin loop of the iron responsive element (IRE) in ferritin mRNA for structure: an NMR spectroscopy study.Nucleic acids research · 1995Article
- Iron regulatory elements (IREs): a family of mRNA non-coding sequences.The Biochemical journal · 1994Review
- Translational enhancement of H-ferritin mRNA by interleukin-1 beta acts through 5' leader sequences distinct from the iron responsive element.Nucleic acids research · 1994Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The ferritin iron regulatory element (IRE), a conserved sequence of 28 nucleotides in a hairpin loop, is a conserved mRNA-specific translational regulatory element; flanking the IRE are regions of varying sequence, which form 9-17 base pairs close to the 5' cap. P-90 is a ferritin mRNA-specific translation regulatory protein purified from animal liver and reticulocytes. To study the P-90-RNA interaction, protein nucleases (RNase S1 and T1) and chemical nucleases FeEDTA and/or 1,10-phenanthroline-Cu were used as probes of an oligonucleotide (n = 55), containing the IRE and flanking regions (FL), and natural ferritin mRNA. Footprints and "toeprints" showed that P-90 binding was confined to the stem and loop of the IRE itself. However, P-90 altered the structure of the flanking region by increasing base stacking or helicity (RNase V1 sensitivity). Comparison of the reactivity of the IRE and flanking regions in natural mRNA and the 55-mer showed that long-range interactions included protecting bulges, single-stranded, and stacked regions from protein nucleases as well as stabilizing the P-90-RNA interaction. Structural integration of the IRE with the base-paired flanking regions was indicated by common features of reactivity (periodic hypersensitivity to FeEDTA) and changes in the FL region caused by P-90. The increased secondary structure of the IRE flanking regions caused by P-90 binding to the IRE provides a likely mechanism for blocking initiation of ferritin mRNA translation, since the combined structure (IRE + FL) is so close (8-17 nucleotides) to the cap.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.