ArticleGenes & nutrition2008
Validating nutrient-related gene expression changes from microarrays using RT(2) PCR-arrays.
Article in Genes & nutrition, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Adipose tissue gene expression is differentially regulated with different rates of weight loss in overweight and obese humans.International journal of obesity (2005) · 2017Trial
- Single Platform for Gene and Protein Expression Analyses Using Luminescent Gold Nanoclusters.ACS omega · 2018Article
- Iron deficiency anemia and glucose metabolism.Acta bio-medica : Atenei Parmensis · 2017Review
- Article
- Interaction of key pathways in sorafenib-treated hepatocellular carcinoma based on a PCR-array.International journal of clinical and experimental pathology · 2015Article
- Article
- Comparisons of the iron deficient metabolic response in rats fed either an AIN-76 or AIN-93 based diet.Nutrition & metabolism · 2012Article
- ReadqPCR and NormqPCR: R packages for the reading, quality checking and normalisation of RT-qPCR quantification cycle (Cq) data.BMC genomics · 2012Article
- Enhanced expression of lipogenic genes may contribute to hyperglycemia and alterations in plasma lipids in response to dietary iron deficiency.Genes & nutrition · 2012Article
- Processes underlying the nutritional programming of embryonic development by iron deficiency in the rat.PloS one · 2012Article
- Systems biology approach to identify transcriptome reprogramming and candidate microRNA targets during the progression of polycystic kidney disease.BMC systems biology · 2011Article
- Cell cycle regulation and cytoskeletal remodelling are critical processes in the nutritional programming of embryonic development.PloS one · 2011Article
- Customized PCR-array analysis informed by gene-chip microarray and biological hypothesis reveals pathways involved in lung inflammatory response to titanium dioxide in pregnancy.Journal of toxicology and environmental health. Part A · 2010Article
- Use of microarray hybridization to identify Brugia genes involved in mosquito infectivity.Parasitology research · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microarray technology allows us to perform high-throughput screening of changes in gene expression. The outcome of microarray experiments largely depends on the applied analysis methods and cut-off values chosen. Results are often required to be verified using a more sensitive detection technique, such as quantitative real-time PCR (qPCR or RT-PCR). Throughout the years, this technique has become a de facto golden standard. Individual qPCRs are time-consuming, but the technology to perform high-throughput qPCR reactions has become available through PCR-arrays that allow up to 384 PCR reactions simultaneously. Our current aim was to investigate the usability of a RT(2) Profilertrade mark PCR-array as validation in a nutritional intervention study, where the measured changes in gene expression were low. For some differentially expressed genes, the PCR-array confirmed the microarray prediction, though not for all. Furthermore, the PCR-array allowed picking up the expression of genes that were not measurable on the microarray platform but also vice versa. We conclude that both techniques have their own (dis)advantages and specificities, and for less pronounced changes using both technologies may be useful as complementation rather than validation.
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Registered trials
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