ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2009
Exploration and mapping of microsatellite markers from subtracted drought stress ESTs in Sorghum bicolor (L.) Moench.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 54 citations in OpenAlex.
- Recent advancements in the breeding of sorghum crop: current status and future strategies for marker-assisted breeding.Frontiers in genetics · 2023Review
- Occurrence of simple sequence repeats in cDNA sequences of safflower (Frontiers in plant science · 2022Article
- De novo identification and targeted sequencing of SSRs efficiently fingerprints Sorghum bicolor sub-population identity.PloS one · 2021Article
- Genetic structure and diversity analysis of tall fescue populations by EST-SSR and ISSR markers.Molecular biology reports · 2020Article
- Milestones achieved in response to drought stress through reverse genetic approaches.F1000Research · 2018Review
- Validation of QTL mapping and transcriptome profiling for identification of candidate genes associated with nitrogen stress tolerance in sorghum.BMC plant biology · 2017Article
- Mapping QTLs and association of differentially expressed gene transcripts for multiple agronomic traits under different nitrogen levels in sorghum.BMC plant biology · 2016Article
- Detection and validation of stay-green QTL in post-rainy sorghum involving widely adapted cultivar, M35-1 and a popular stay-green genotype B35.BMC genomics · 2014Article
- Mapping QTL for grain yield and other agronomic traits in post-rainy sorghum [Sorghum bicolor (L.) Moench].TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Assessment of genetic diversity in the sorghum reference set using EST-SSR markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Heterotic trait locus (HTL) mapping identifies intra-locus interactions that underlie reproductive hybrid vigor in Sorghum bicolor.PloS one · 2012Article
- QTL for fibre-related traits in grain × sweet sorghum as a tool for the enhancement of sorghum as a biomass crop.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Identification and validation of genomic regions that affect shoot fly resistance in sorghum [Sorghum bicolor (L.) Moench].TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Genetic mapping of QTLs for sugar-related traits in a RIL population of Sorghum bicolor L. Moench.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Identification of quantitative trait loci for resistance to shoot fly in sorghum [Sorghum bicolor (L.) Moench].TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Identification of quantitative trait loci for agronomically important traits and their association with genic-microsatellite markers in sorghum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Molecular variation within defined genes underlying specific biochemical or physiological functions provide candidate gene-based markers which show very close association with the trait of interest and thus should enable to design superior genotypes. We explored microsatellite loci in a total of 9,892 subtracted drought stress ESTs of sorghum (6,295 after flowering ESTs and 3,597 before flowering ESTs) available in the NCBI dbEST database. Analysis of 9,892 ESTs identified 221 non-redundant ESTs with SSRs, from which 109 functional SSRs were developed. Among them 62 EST-microsatellites (56.8%) exhibited polymorphism for at least one sorghum genotype among the five tested and yielded a total of 161 alleles, with an average of 2.59 alleles per marker. We present a microsatellite linkage map using a RIL population derived from the cross 296B and IS18551. The map contains 128 microsatellite loci distributed over 15 linkage groups, and spanning a genetic distance of 1,074.5 cM. The map includes map positions of 28 drought EST-microsatellites developed and seven new genomic-SSRs, and are distributed throughout the map. The developed EST markers include genes coding for important regulatory proteins and functional proteins that are involved in stress related metabolism. The drought EST-microsatellites will have applications in functional diversity studies, association studies, QTL studies for drought, and other agronomically important traits in sorghum, and comparative genomics studies between sorghum and other members of the Poaceae family.
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