ArticleMolecular therapy : the journal of the American Society of Gene Therapy2009
Analysis of adeno-associated virus progenitor cell transduction in mouse lung.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- AAV-mediated gene editing lights up the lung.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- Tissue and cell-type-specific transduction using rAAV vectors in lung diseases.Journal of molecular medicine (Berlin, Germany) · 2021Review
- Targeted Gene Delivery through the Respiratory System: Rationale for Intratracheal Gene Transfer.Journal of cardiovascular development and disease · 2019Review
- Adeno-Associated Virus (AAV) gene therapy for cystic fibrosis: current barriers and recent developments.Expert opinion on biological therapy · 2017Review
- Cystic Fibrosis Gene Therapy in the UK and Elsewhere.Human gene therapy · 2015Review
- Stem cells, cell therapies, and bioengineering in lung biology and diseases. Comprehensive review of the recent literature 2010-2012.Annals of the American Thoracic Society · 2013Review
- Gene delivery to the airway.Current protocols in human genetics · 2013Review
- A vector-host system to fingerprint virus tropism.Human gene therapy · 2012Article
- The a"MAZE"ing world of lung-specific transgenic mice.American journal of respiratory cell and molecular biology · 2012Review
- Jaagsiekte sheep retrovirus pseudotyped lentiviral vector-mediated gene transfer to fetal ovine lung.Gene therapy · 2012Article
- Immunohistochemical demonstration of airway epithelial cell markers of guinea pig.Tissue & cell · 2011Article
- Stem cells and cell therapies in lung biology and lung diseases.Proceedings of the American Thoracic Society · 2011Article
- Genetic therapies for cystic fibrosis lung disease.Human molecular genetics · 2011Review
- Genetic modification of airway progenitors after lentiviral gene delivery to the amniotic fluid of murine fetuses.American journal of respiratory cell and molecular biology · 2011Article
- Clinical review: gene-based therapies for ALI/ARDS: where are we now?Critical care (London, England) · 2011Review
- Intersection of gene therapy and progenitor cell biology in the lung.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Although recombinant adeno-associated virus (rAAV) has been widely used in lung gene therapy approaches, it remains unclear to what extent commonly used AAV serotypes transduce adult progenitors in the lung. In this study, we evaluated the life span and proliferative capacity of rAAV1-, 2-, and 5-transduced airway cells in mouse lung, using a LacZ-CRE reporter transgenic model and Cre-expressing rAAV. In this model, the expression of CRE recombinase led to permanent genetic marking of transduced cells and their descendants with LacZ. To investigate whether the rAAV-transduced cells included airway progenitors, we injured the airways of rAAV-infected mice with Naphthalene, while simultaneously labeling with 5-bromodeoxyuridine (BrdU) to identify slow-cycling progenitor/stem cells that entered the cell cycle and retained label. Both rAAV5 and rAAV1 vectors were capable of transducing a subset of long-lived Clara cells and alveolar type II (ATII) cells that retained nucleotide label and proliferated following lung injury. Importantly, rAAV1 and 5 appeared to preferentially transduce conducting airway epithelial progenitors that had the capacity to clonally expand, both in culture and in vivo following lung injury. These studies suggest that rAAV may be a useful vector for gene targeting of airway stem/progenitor cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.