ArticleJournal of experimental botany2009
Metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to Zn accumulation in the metal hyperaccumulator, Thlaspi caerulescens.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 92 citations in OpenAlex.
- Effects of Zinc on Metallicolous and Non-Metallicolous Populations ofPlants (Basel, Switzerland) · 2025Article
- The Contribution ofFoods (Basel, Switzerland) · 2024Article
- Metallothionein family genes in kiwifruit: characterization and determining their roles in plant's response to different stresses.Frontiers in plant science · 2024Article
- Genome-wide analysis of the metallothionein gene family in cassava reveals its role in response to physiological stress through the regulation of reactive oxygen species.BMC plant biology · 2023Article
- Exogenous hydrogen sulfide and methylglyoxal alleviate cadmium-induced oxidative stress in Salix matsudana Koidz by regulating glutathione metabolism.BMC plant biology · 2023Article
- Phytoremediation of Potentially Toxic Elements: Role, Status and Concerns.Plants (Basel, Switzerland) · 2023Review
- Genome-wide analysis of metallothionein gene family in maize to reveal its role in development and stress resistance to heavy metal.Biological research · 2022Article
- Article
- Differences of Cd uptake and expression of MT family genes and NRAMP2 in two varieties of ryegrasses.Environmental science and pollution research international · 2019Article
- Necrotrophic fungus Macrophomina phaseolina tolerates chromium stress through regulating antioxidant enzymes and genes expression (MSN1 and MT).Environmental science and pollution research international · 2019Article
- Characterization analysis and heavy metal-binding properties ofFEBS open bio · 2018Article
- Genome-Wide Characterization and Analysis of Metallothionein Family Genes That Function in Metal Stress Tolerance inInternational journal of molecular sciences · 2018Article
- Functional characterization of a type 2 metallothionein gene, SsMT2, from alkaline-tolerant Suaeda salsa.Scientific reports · 2017Article
- Article
- Gain-of-function inSpringerPlus · 2016Article
- Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes.Protoplasma · 2014Article
- Differential responses of three sweetpotato metallothionein genes to abiotic stress and heavy metals.Molecular biology reports · 2014Article
- Abiotic stress response in yeast and metal-binding ability of a type 2 metallothionein-like protein (PutMT2) from Puccinellia tenuiflora.Molecular biology reports · 2014Article
- The SbMT-2 gene from a halophyte confers abiotic stress tolerance and modulates ROS scavenging in transgenic tobacco.PloS one · 2014Article
- Metallothionein 2 (SaMT2) from Sedum alfredii Hance confers increased Cd tolerance and accumulation in yeast and tobacco.PloS one · 2014Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study the role of metallothioneins (MTs) in Zn accumulation, the expression of TcMT2a, TcMT2b, and TcMT3 was analysed in three accessions and 15 F(3) families of two inter-accession crosses of the Cd/Zn hyperaccumulator Thlaspi caerulescens, with different degrees of Zn accumulation. The highest expression levels were found in the shoots of a superior metal-accumulating calamine accession from St Laurent le Minier, with >10-fold TcMT3 expression compared with another calamine accession and a non-metallicolous accession. Moreover, F(3) sibling lines from the inter-accession crosses that harboured the MT2a or MT3 allele from St Laurent le Minier had higher expression levels. However, there was no co-segregation of TcMT2a or TcMT3 expression and Zn accumulation. To examine the functions of TcMTs in plants, TcMT2a and TcMT3 were ectopically expressed in Arabidopsis. The transformant lines had reduced root length in control medium but not at high metal concentrations, suggesting that the ectopically expressed proteins interfered with the physiological availability of essential metals under limited supply. The Arabidopsis transformant lines did not show increased tolerance to Cd, Cu, or Zn, nor increased Cd or Zn accumulation. Immunohistochemical analysis indicated that in roots, MT2 protein is localized in the epidermis and root hairs of both T. caerulescens and Arabidopsis thaliana. The results suggest that TcMT2a, TcMT2b, and TcMT3 are not primarily involved in Zn accumulation as such. However, the elevated expression levels in the metallicolous accessions suggests that they do contribute to the metal-adapted phenotype, possibly through improving Cu homeostasis at high Zn and Cd body burdens. Alternatively, they might function as hypostatic enhancers of Zn or Cd tolerance.
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