ArticleJournal of experimental botany2009
GAL4 GFP enhancer trap lines for analysis of stomatal guard cell development and gene expression.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 87 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Enhancers in Plant Development, Adaptation and Evolution.Plant & cell physiology · 2025Review
- An enhancer trap system to track developmental dynamics in Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2023Article
- The design of synthetic gene circuits in plants: new components, old challenges.Journal of experimental botany · 2023Review
- Leaf vein patterning is regulated by the aperture of plasmodesmata intercellular channels.PLoS biology · 2022Article
- Leaf cell-specific and single-cell transcriptional profiling reveals a role for the palisade layer in UV light protection.The Plant cell · 2022Article
- Cis-regulatory sequences in plants: Their importance, discovery, and future challenges.The Plant cell · 2022Review
- Design of orthogonal regulatory systems for modulating gene expression in plants.Nature chemical biology · 2020Article
- Combining GAL4 GFP enhancer trap with split luciferase to measure spatiotemporal promoter activity in Arabidopsis.The Plant journal : for cell and molecular biology · 2020Article
- Genetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis and Maize.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Synthetic Switches and Regulatory Circuits in Plants.Plant physiology · 2019Review
- Molecular identification of a root apical cell-specific and stress-responsive enhancer from anPlant methods · 2019Article
- Synthetic Botany.Cold Spring Harbor perspectives in biology · 2017Review
- Immunity at Cauliflower Hydathodes Controls Systemic Infection byPlant physiology · 2017Article
- Differential fine-tuning of gene expression regulation in coffee leaves by CcDREB1D promoter haplotypes under water deficit.Journal of experimental botany · 2017Article
- Molecular Characterization of Arabidopsis GAL4/UAS Enhancer Trap Lines Identifies Novel Cell-Type-Specific Promoters.Plant physiology · 2016Article
- NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis.Development (Cambridge, England) · 2016Article
- Meselect - A Rapid and Effective Method for the Separation of the Main Leaf Tissue Types.Frontiers in plant science · 2016Article
- Guard cell photosynthesis is critical for stomatal turgor production, yet does not directly mediate CO2 - and ABA-induced stomatal closing.The Plant journal : for cell and molecular biology · 2015Article
- Transcriptome dynamics of the stomatal lineage: birth, amplification, and termination of a self-renewing population.Developmental cell · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
To facilitate the monitoring of guard cells during development and isolation, a population of 704 GAL4 GFP enhancer trap lines was screened and four single insert lines with guard cell GFP expression and one with developmentally-regulated guard cell GFP expression were identified. The location of the T-DNA inserts, the expression of the flanking genes, and the promoter activity of the genomic DNA upstream of the T-DNA were characterized. The results indicated that the GFP expression pattern in at least one of the lines was due to elements in the intergenic DNA immediately upstream of the T-DNA, rather than due to the activity of the promoters of genes flanking the insert, and provide evidence for the involvement of Dof elements in regulating guard cell gene expression. It is shown further that the GAL4 GFP lines can be used to track the contribution of guard cell material in vitro, and this method was used to assess the purity of guard cell samples obtained using two methods of guard cell isolation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.