ArticleThe Plant cell2008
Arabidopsis phosphatidylinositol-4-monophosphate 5-kinase 4 regulates pollen tube growth and polarity by modulating membrane recycling.
Article in The Plant cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 154 citations in OpenAlex.
- The class VIII myosin ATM2 stabilizes actin-plasma membrane contacts in tobacco pollen tubes by locally promoting PtdIns(4,5)PNature plants · 2026Article
- Students' knowledge of plant anatomy and physiology as a reflection of interest in the teaching profession.Scientific reports · 2026Article
- Reciprocal regulation between AtFH5-labeled secretory vesicles and PI(4,5)PJournal of integrative plant biology · 2025Article
- Plant PI-PLC signaling in stress and development.Plant physiology · 2025Review
- Imaging Plant Lipids with Fluorescent Reporters.Plants (Basel, Switzerland) · 2024Review
- Article
- OsPIPK-FAB, A Negative Regulator in Rice Immunity Unveiled by OsMBL1 Inhibition.Rice (New York, N.Y.) · 2024Article
- Genetic interaction between TTG2 and AtPLC1 reveals a role for phosphoinositide signaling in a co-regulated suite of Arabidopsis epidermal pathways.Scientific reports · 2024Article
- Genome-wide identification and characterization of NAC transcription factor family members in Trifolium pratense and expression analysis under lead stress.BMC genomics · 2024Article
- Phosphoinositides in plant-pathogen interaction: trends and perspectives.Stress biology · 2023Review
- The Light-Controlled Release of 2-fluoro-l-fucose, an Inhibitor of the Root Cell Elongation, from a nitrobenzyl-caged Derivative.International journal of molecular sciences · 2023Article
- Descriptive Genomic Analysis and Sequence Genotyping of the Two Papaya Species (Vasconcellea pubescens and Vasconcellea chilensis) Using GBS Tools.Plants (Basel, Switzerland) · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- Towards engineering ectomycorrhization into switchgrass bioenergy crops via a lectin receptor-like kinase.Plant biotechnology journal · 2021Article
- Plasma membrane nano-organization specifies phosphoinositide effects on Rho-GTPases and actin dynamics in tobacco pollen tubes.The Plant cell · 2021Article
- Investigating the Mechanism of Unilateral Cross Incompatibility in Longan (Frontiers in plant science · 2021Article
- Lipid kinases PIP5K7 and PIP5K9 are required for polyamine-triggered KThe Plant journal : for cell and molecular biology · 2020Article
- Signalling Pinpointed to the Tip: The Complex Regulatory Network That Allows Pollen Tube Growth.Plants (Basel, Switzerland) · 2020Review
- Quantitative Structural Organization of Bulk Apical Membrane Traffic in Pollen Tubes.Plant physiology · 2020Article
- Specific Recruitment of Phosphoinositide Species to the Plant-Pathogen Interfacial Membrane Underlies Arabidopsis Susceptibility to Fungal Infection.The Plant cell · 2020Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylinositol-4-monophosphate 5-kinases produce phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)P(2)] and have been implicated in vesicle trafficking and cytoskeletal rearrangements. Here, we adopted a reverse genetics approach to investigate the function of the Arabidopsis thaliana pollen-expressed gene encoding phosphatidylinositol-4-monophosphate 5-kinase 4 (PIP5K4). Pollen germination, tube growth, and polarity were significantly impaired in homozygous mutant plants lacking PIP5K4 transcript. In vitro, supplementation with PtdIns(4,5)P(2) rescued these phenotypes. In vivo, mutant pollen fertilized ovules, leading to normal seed set and silique length. However, fertilization took longer than in wild-type plants, and the pip5k4 null mutant allele was transmitted through the pollen at a reduced frequency. Analysis of endocytic events using FM1-43 (or FM4-64) suggested a reduction in endocytosis and membrane recycling in pip5k4 null mutant pollen tubes. Imaging of elongating tobacco (Nicotiana tabacum) pollen tubes transiently transformed with a PIP5K4-green fluorescent protein fusion construct revealed that the protein localized to the plasma membrane, particularly in the subapical region. Overexpression of PIP5K4-GFP delocalized the protein to the apical region of the plasma membrane, perturbed pollen tube growth, and caused apical cell wall thickening. Thus, PIP5K4 plays a crucial role in regulating the polarity of pollen tubes. This study supports a model for membrane secretion and recycling where the apical and subapical regions appear to contain the components required to promote and sustain growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.