ArticleProceedings of the National Academy of Sciences of the United States of America2008
Termination codon-dependent translation of partially overlapping ndhC-ndhK transcripts in chloroplasts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Translational coupling of neighboring genes in prokaryotes.Journal of bacteriology · 2025Review
- Transgene insertion into the plastid genome alters expression of adjacent native chloroplast genes at the transcriptional and translational levels.Plant biotechnology journal · 2023Article
- Chloroplast Translation: Structural and Functional Organization, Operational Control, and Regulation.The Plant cell · 2018Review
- Variation and evolution of polyadenylation profiles in sauropsid mitochondrial mRNAs as deduced from the high-throughput RNA sequencing.BMC genomics · 2017Article
- Comparative analysis of complete plastid genomes from wild soybean (Glycine soja) and nine other Glycine species.PloS one · 2017Article
- Seamless editing of the chloroplast genome in plants.BMC plant biology · 2016Article
- Additional pathway to translate the downstream ndhK cistron in partially overlapping ndhC-ndhK mRNAs in chloroplasts.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The complete sequence of the mitochondrial genome of Butomus umbellatus--a member of an early branching lineage of monocotyledons.PloS one · 2013Article
- Translation of partially overlapping psbD-psbC mRNAs in chloroplasts: the role of 5'-processing and translational coupling.Nucleic acids research · 2012Article
- The downstream atpE cistron is efficiently translated via its own cis-element in partially overlapping atpB-atpE dicistronic mRNAs in chloroplasts.Nucleic acids research · 2011Article
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2 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The chloroplast NAD(P)H dehydrogenase complex, a homologue of mitochondrial complex I, consists of >15 subunits, of which 11 are encoded by the chloroplast genome (ndhA-K). The ndhC and ndhK genes are partially overlapped and cotranscribed in many land plants. The downstream ndhK mRNA possesses 4 possible AUG initiation codons in many dicot plants. By using an efficient in vitro translation system from tobacco chloroplasts, we defined that the major initiation site of tobacco ndhK mRNAs is the third AUG that is located 4 nt upstream from the ndhC stop codon. Mutation of the ndhC stop codon (UAG) arrested translation of the ndhK cistron. Frameshift of the ndhC coding strand inhibited also translation of the distal cistron. The results indicated that ndhK translation depends on termination of the preceding cistron, namely translational coupling. Surprisingly, removal of the ndhC 5'-UTR and its AUG still supported substantial translation of the ndhK cistron. This translation was abolished again by removing the ndhC stop codon. Although translation of the downstream cistron of an overlapping mRNA is generally very low, we found that the ndhC/K mRNA produces NdhK and NdhC in similar amounts. Based on subunit compositions of the bacterial complex I, the stoichiometry of NdhK and NdhC is suggested to be 1:1 in chloroplasts. To meet this stoichiometry, the ndhC/K mRNA is translated not only by a translational coupling event but also by a termination codon-dependent pathway.
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