ArticleNucleic acids research2009
The class E floral homeotic protein SEPALLATA3 is sufficient to loop DNA in 'floral quartet'-like complexes in vitro.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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68 citing papers in PubMed.
- Genome-wide characterization of MADS-box genes reveals CsaSOC1 as a key regulator of flowering in Cannabis sativa L.Plant cell reports · 2026Article
- A novel CsbZIP26-CsSEP4-CsSPL18 regulatory module governs gynostemium morphology and floral architecture inHorticulture research · 2026Article
- Article
- Targets and mechanisms of epigenetic regulation in the temperate cereal vernalisation process.Frontiers in plant science · 2025Review
- Identification and characterization of two APETALA2 homolog genes in lotus (Nelumbo nucifera) involved in sepal and petal development.BMC plant biology · 2024Article
- Single nucleotide polymorphisms in SEPALLATA 2 underlie fruit length variation in cucurbits.The Plant cell · 2024Article
- SEPALLATA-driven MADS transcription factor tetramerization is required for inner whorl floral organ development.The Plant cell · 2024Article
- Reflections on the ABC model of flower development.The Plant cell · 2024Review
- Cell layer-specific expression of the homeotic MADS-box transcription factor PhDEF contributes to modular petal morphogenesis in petunia.The Plant cell · 2024Article
- AGAMOUS mediates timing of guard cell formation during gynoecium development.PLoS genetics · 2023Article
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2023Article
- The Origin of Floral Quartet Formation-Ancient Exon Duplications Shaped the Evolution of MIKC-type MADS-domain Transcription Factor Interactions.Molecular biology and evolution · 2023Article
- Flower Development in Arabidopsis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- The MADS-box gene FveSEP3 plays essential roles in flower organogenesis and fruit development in woodland strawberry.Horticulture research · 2021Article
- The intervening domain is required for DNA-binding and functional identity of plant MADS transcription factors.Nature communications · 2021Article
- Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron.Scientific reports · 2021Article
- Genome-wide binding of SEPALLATA3 and AGAMOUS complexes determined by sequential DNA-affinity purification sequencing.Nucleic acids research · 2020Article
- Characterization of Transcriptional Expression and Regulation of Carotenoid Cleavage Dioxygenase 4b in Grapes.Frontiers in plant science · 2020Article
- Divergent Functional Diversification Patterns in the SEP/AGL6/AP1 MADS-Box Transcription Factor Superclade.The Plant cell · 2019Article
8 more citing papers are in PubMed but not listed here.
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3 authors.
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Abstract
The organs of a eudicot flower are specified by four functional classes, termed class A, B, C and E, of MADS domain transcription factors. The combinatorial formation of tetrameric complexes, so called 'floral quartets', between these classes is widely believed to represent the molecular basis of floral organ identity specification. As constituents of all complexes, the class E floral homeotic proteins are thought to be of critical relevance for the formation of floral quartets. However, experimental support for tetrameric complex formation remains scarce. Here we provide physico-chemical evidence that in vitro homotetramers of the class E floral homeotic protein SEPALLATA3 from Arabidopsis thaliana bind cooperatively to two sequence elements termed 'CArG boxes' in a phase-dependent manner involving DNA looping. We further show that the N-terminal part of SEPALLATA3 lacking K3, a subdomain of the protein-protein interactions mediating K domain, and the C-terminal domain, is sufficient for protein dimerization, but not for tetramer formation and cooperative DNA binding. We hypothesize that the capacity of class E MADS domain proteins to form tetrameric complexes contributes significantly to the formation of floral quartets. Our findings further suggest that the spacing and phasing of CArG boxes are important parameters in the molecular mechanism by which floral homeotic proteins achieve target gene specificity.
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