ArticleTransgenic research2009
Production of recombinant albumin by a herd of cloned transgenic cattle.
Article in Transgenic research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- High-Yield Preparation and Characterization of Feline Albumin with Antioxidant Properties and In Vivo Safety.International journal of molecular sciences · 2024Article
- Outlook on genome editing application to cattle.Journal of veterinary science · 2024Review
- Cows as Bioreactors for the Production of Nutritionally and Biomedically Significant Proteins.Advances in experimental medicine and biology · 2022Article
- Efficient correction of a deleterious point mutation in primary horse fibroblasts with CRISPR-Cas9.Scientific reports · 2020Article
- Structural and functional characterization of recombinant human growth hormone isolated from transgenic pig milk.PloS one · 2020Article
- Production of transgenic cattle expressing lysine-rich polypeptide in milk by somatic cell nuclear transfer.Transgenic research · 2019Article
- Efficient targeted integration into the bovine Rosa26 locus using TALENs.Scientific reports · 2018Article
- Article
- Revisiting Escherichia coli as microbial factory for enhanced production of human serum albumin.Microbial cell factories · 2017Article
- Large-scale production of recombinant human lactoferrin from high-expression, marker-free transgenic cloned cows.Scientific reports · 2017Article
- Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules.Human vaccines & immunotherapeutics · 2017Review
- The transgenic animal platform for biopharmaceutical production.Transgenic research · 2016Review
- Expression of Active Fluorophore Proteins in the Milk of Transgenic Pigs Bypassing the Secretory Pathway.Scientific reports · 2016Article
- Transgenic bovine as bioreactors: Challenges and perspectives.Bioengineered · 2016Review
- Generation of TALE nickase-mediated gene-targeted cows expressing human serum albumin in mammary glands.Scientific reports · 2016Article
- Production of transgenic cattle highly expressing human serum albumin in milk by phiC31 integrase-mediated gene delivery.Transgenic research · 2015Article
- Engineering protein processing of the mammary gland to produce abundant hemophilia B therapy in milk.Scientific reports · 2015Article
- TALEN-mediated modification of the bovine genome for large-scale production of human serum albumin.PloS one · 2014Article
- Identification of abnormal gene expression in bovine transgenic somatic cell nuclear transfer embryos.Journal of veterinary science · 2014Article
- Use of transgenic animals in biotechnology: prospects and problems.Acta naturae · 2013Article
Corrections and comments
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Authors and funding
15 authors at 3 institutions in 2 countries.
Funding
Abstract
Purified plasma derived human albumin has been available as a therapeutic product since World War II. However, cost effective recombinant production of albumin has been challenging due to the amount needed and the complex folding pattern of the protein. In an effort to provide an abundant source of recombinant albumin, a herd of transgenic cows expressing high levels of rhA in their milk was generated. Expression cassettes efficiently targeting the secretion of human albumin to the lactating mammary gland were obtained and tested in transgenic mice. A high expressing transgene was transfected in primary bovine cell lines to produce karyoplasts for use in a somatic cell nuclear transfer program. Founder transgenic cows were produced from four independent cell lines. Expression levels varying from 1-2 g/l to more than 40 g/l of correctly folded albumin were observed. The animals expressing the highest levels of rhA exhibited shortened lactation whereas cows yielding 1-2 g/l had normal milk production. This herd of transgenic cattle is an easily scalable and well characterized source of rhA for biomedical uses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.