ArticlePloS one2008
Differential roles of the PKC novel isoforms, PKCdelta and PKCepsilon, in mouse and human platelets.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- TNIK as a molecular switch regulating platelet function in hemostasis and hyperlipidemia-associated thrombosis.Blood advances · 2026Article
- Impact of sampling technique, anticoagulant, processing delay, and temperature on murine platelet function in whole blood.Research and practice in thrombosis and haemostasis · 2025Article
- Experimental conditions shapeFrontiers in immunology · 2025Article
- Protein kinase C and endothelial dysfunction in select vascular diseases.Frontiers in cardiovascular medicine · 2025Review
- Modulation of Glycoprotein VI and Its Downstream Signaling Pathways as an Antiplatelet Target.International journal of molecular sciences · 2022Review
- mTOR regulates GPVI-mediated platelet activation.Journal of translational medicine · 2021Article
- Combination of Platelet expression of PKCepsilon and cardiac troponin-I for early diagnosis of chest pain patients in the emergency department.Scientific reports · 2019Article
- Human Platelet Protein Ubiquitylation and Changes following GPVI Activation.Thrombosis and haemostasis · 2019Article
- Role of Protein Kinase C-delta in regulating platelet activation and platelet-leukocyte interaction during sepsis.PloS one · 2018Article
- Platelet expression of PKCepsilon oncoprotein in myelofibrosis is associated with disease severity and thrombotic risk.Annals of translational medicine · 2017Article
- Human thrombopoiesis depends on Protein kinase Cδ/protein kinase Cε functional couple.Haematologica · 2016Article
- IL-32-PAR2 axis is an innate immunity sensor providing alternative signaling for LPS-TRIF axis.Scientific reports · 2013Article
- Tyrosine phosphorylation on spleen tyrosine kinase (Syk) is differentially regulated in human and murine platelets by protein kinase C isoforms.The Journal of biological chemistry · 2013Article
- Protein kinase Cε and protein kinase Cθ double-deficient mice have a bleeding diathesis.Journal of thrombosis and haemostasis : JTH · 2012Article
- Protein kinase C isoform ε negatively regulates ADP-induced calcium mobilization and thromboxane generation in platelets.Arteriosclerosis, thrombosis, and vascular biology · 2012Article
- Protein kinase C ε expression in platelets from patients with acute myocardial infarction.PloS one · 2012Article
- Protein kinase Cδ mediates the activation of protein kinase D2 in platelets.Biochemical pharmacology · 2011Article
- Defining protein kinase/phosphatase isoenzymic regulation of mGlu₅ receptor-stimulated phospholipase C and Ca²⁺ responses in astrocytes.British journal of pharmacology · 2011Article
- Submaximal inhibition of protein kinase C restores ADP-induced dense granule secretion in platelets in the presence of Ca2+.The Journal of biological chemistry · 2011Article
- Platelet protein kinase C-theta deficiency with human RUNX1 mutation: PRKCQ is a transcriptional target of RUNX1.Arteriosclerosis, thrombosis, and vascular biology · 2011Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIncreasing evidence suggests that individual isoforms of protein kinase C (PKC) play distinct roles in regulating platelet activation. METHODOLOGY/PRINCIPAL
findingsIn this study, we focus on the role of two novel PKC isoforms, PKCdelta and PKCepsilon, in both mouse and human platelets. PKCdelta is robustly expressed in human platelets and undergoes transient tyrosine phosphorylation upon stimulation by thrombin or the collagen receptor, GPVI, which becomes sustained in the presence of the pan-PKC inhibitor, Ro 31-8220. In mouse platelets, however, PKCdelta undergoes sustained tyrosine phosphorylation upon activation. In contrast the related isoform, PKCepsilon, is expressed at high levels in mouse but not human platelets. There is a marked inhibition in aggregation and dense granule secretion to low concentrations of GPVI agonists in mouse platelets lacking PKCepsilon in contrast to a minor inhibition in response to G protein-coupled receptor agonists. This reduction is mediated by inhibition of tyrosine phosphorylation of the FcRgamma-chain and downstream proteins, an effect also observed in wild-type mouse platelets in the presence of a PKC inhibitor.
conclusionsThese results demonstrate a reciprocal relationship in levels of the novel PKC isoforms delta and epsilon in human and mouse platelets and a selective role for PKCepsilon in signalling through GPVI.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.