ArticleAntimicrobial agents and chemotherapy2009
qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovismorbificans strains of human origin.
Article in Antimicrobial agents and chemotherapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 178 papers, 1 of them a synthesis that pooled it.
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178 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Plasmid-mediated quinolone resistance in Enterobacteriaceae: a systematic review with a focus on Mediterranean countries.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Pooled it
- Detection of cefotaxime- and enrofloxacin-resistant and mcr-1-positive Escherichia coli at farm level in younger and older calf groups across different production systems in Germany.BMC microbiology · 2026Article
- Snowmaking Infrastructure: A Selective Barrier or Potential Vector for Antibiotic Residues and Resistance Determinants in Mountain Catchments.International journal of molecular sciences · 2026Article
- Association between phenotypes and genotypes of antimicrobial resistance inMicrobiology spectrum · 2026Article
- Antimicrobial resistance surveillance and focused comparative genomics of clinicalMicrobiology spectrum · 2026Article
- Diversity of third-generation cephalosporin-resistant enterobacterales from human and animal samples obtained in Owerri, Nigeria.BMC infectious diseases · 2026Article
- A Comprehensive Review on Discovery, Development, the Chemistry of Quinolones, and Their Antimicrobial Resistance.Current topics in medicinal chemistry · 2026Review
- Genetic Determinants of Carbapenem and Fluoroquinolone Resistance inInfection & chemotherapy · 2025Article
- Impact of COVID-19 pandemic on antimicrobial resistance of Proteus mirabilis in a Brazilian hospital.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Quinolone Resistance Genes qnr, aac(6')-Ib-cr, oqxAB, and qepA in Environmental Escherichia coli: Insights into Their Genetic Contexts from Comparative Genomics.Microbial ecology · 2025Article
- Unraveling the treasure trove of phytochemicals in mitigating the Salmonella enterica infection.Folia microbiologica · 2025Review
- Epidemiology and Mechanism of Drug Resistance of Multidrug-Resistant Klebsiella Pneumoniae Isolated from Patients with Urinary Tract Infection in Beijing Teaching Hospital, China.Infection and drug resistance · 2025Article
- Genomic features ofFrontiers in veterinary science · 2025Article
- Prevalence of Plasmid-Mediated Quinolone Resistance Genes inHealth science reports · 2024Article
- Molecular mechanisms impact on fluoroquinolone resistance among E.coli from enteric carriage monitoring before prostate biopsy and earliest description of qnrB81.Scientific reports · 2024Article
- Occurrence of Plasmid-Mediated Quinolone Resistance and Carbapenemase-Encoding Genes inPathogens (Basel, Switzerland) · 2024Observational
- Genomic Characterization of 16S rRNA Methyltransferase-ProducingAntibiotics (Basel, Switzerland) · 2024Article
- Correlation analysis of whole genome sequencing of a pathogenic Escherichia coli strain of Inner Mongolian origin.Scientific reports · 2024Article
- Genomic Characterization of Carbapenemase-ProducingAntibiotics (Basel, Switzerland) · 2024Article
- Salmonella enterica serovars linked with poultry in India: antibiotic resistance profiles and carriage of virulence genes.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
118 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
In a previous study, four Salmonella isolates from humans in the Henan province of China showed reduced susceptibility to ciprofloxacin (MIC, 0.125 to 0.25 microg/ml) but were susceptible to nalidixic acid (MIC, 4 to 8 microg/ml). All isolates were negative for known qnr genes (A, B, and S), aac(6')Ib-cr, and mutations in gyrA and parC. Plasmid DNA was extracted from all four isolates and transformed into Escherichia coli TG1 and DH10B cells by electroporation, and transformants were selected on 0.06 microg/ml ciprofloxacin containing brain heart infusion agar plates. Resistance to ciprofloxacin could be transferred by electroporation, and a similar 4,270-bp plasmid was found in all transformants. By sequence analysis, the plasmid was found to carry an open reading frame that had similarities to other qnr genes and that encoded a 214-amino-acid pentapeptide repeat protein. This gene, designated qnrD, showed 48% similarity to qnrA1, 61% similarity to qnrB1, and 41% similarity to qnrS1. Further subcloning of the qnrD coding region into the constitutively expressed tetA gene of vector pBR322 showed that the gene conferred an increase in the MIC of ciprofloxacin by a factor of 32 (from an MIC of 0.002 to an MIC of 0.06 microg/ml). For comparison, qnrA1 and qnrS1 were also subcloned into pBR322 and transformed into DH10B cells, conferring MICs of 0.125 and 0.5 microg/ml, respectively. A phylogenetic analysis of all known qnr sequences was performed and showed that qnrD was more closely related to the qnrB variants but formed an independent cluster. To our knowledge, this is the first description of this qnrD gene.
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